| 反义基因逆转肿瘤细胞多药耐药诱导细胞凋亡的研究 |
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| 引用本文： | 张凤春,林玉梅.反义基因逆转肿瘤细胞多药耐药诱导细胞凋亡的研究[J].中华血液学杂志,1997,18(12):627-629. |
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| 作者姓名： | 张凤春 林玉梅 |
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| 作者单位： | 白求恩医科大学第三临床学院血液肿瘤科 |
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| 摘 要： | 目的：探讨克服肿瘤细胞多药耐药（ＭＤＲ）的方法，提高化疗效果。方法：采用多药耐药反义基因（ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ）逆转Ｋ５６２／ＡＤＭ肿瘤细胞的ＭＤＲ，而诱导肿瘤细胞凋亡。结果：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ诱导Ｋ５６２／ＡＤＭ细胞产生大量ＤＮＡ断片，流式细胞仪检测发现几乎全部ｍｄｒ－１＋Ｋ５６２／ＡＤＭ细胞发生凋亡。结论：ｍｄｒ－１－ＡＳＰＳ－ＯＤＮ能有效、特异地抑制ｍｄｒ－１基因表达，逆转肿瘤细胞的ＭＤＲ，促进阿霉素诱导Ｋ５６２／ＡＤＭ细胞凋亡，为其临床应用提供理论依据。
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| 关 键 词： | 反义基因 多药耐药 细胞凋亡 |
Study of antisense oligodeoxynucleotide in reversing multidrug resistance and inducing apoptosis of tumor cells |
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| F Zhang,Y Lin,Y Jiang,X Zhang.Study of antisense oligodeoxynucleotide in reversing multidrug resistance and inducing apoptosis of tumor cells[J].Chinese Journal of Hematology,1997,18(12):627-629. |
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| Authors: | F Zhang Y Lin Y Jiang X Zhang |
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| Institution: | The Third Clinical College, Norman Bethune University of Medical Sciences, Changchun 130021. |
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| Abstract: | OBJECTIVE: T o explore an approach to the reversal of multidrug resistance(MDR) of tumor cells. METHODS: Antisense oligodeoxynucleotide (mdr-1-AS PS-ODN) was used. RESULTS: A large amount of DNA fragments was found in mdr-1-AS PS-ODN treated K562/ADM cells and almost all the mdr-1+ K562/ADM cells underwent apoptosis. CONCLUSION: mdr-1-AS PS-ODN could inhibit specifically mdr-1 gene expression, effectively reverse MDR of the tumor cells and promote ADM induced apoptosis of mdr-1+ K562/ADM. |
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| Keywords: | Antisense oligodeoxynucleotide Multidrug resistance Apoptosis |
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