| 单核细胞的凝血因子活性观察 |
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| 引用本文： | 贾晓梅 郑缓. 单核细胞的凝血因子活性观察[J]. 中华血液学杂志, 1997, 18(9): 474-476 |
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| 作者姓名： | 贾晓梅 郑缓 |
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| 作者单位： | 北京医科大学人民医院血液病研究所!100044(贾晓梅，郑缓，王晶，王逸兰，陆道培)，中国医学科学院中国协和医科大学血液学研究所(李家增) |
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| 摘 要： | 目的：探讨单核细胞的凝血与纤溶活性。方法：将体外培养的28×106单核细胞进行－20℃冷冻，离心（3000r／min，10分钟）后，测定上清液及下层液凝血因子活性，并以2．8×106白细胞上清液作对照。结果：单核细胞上清液有凝血因子Ⅱ、Ⅶ、Ⅸ、Ⅹ、Ⅴ及组织因子活性的表达，并具有特异性。因子（F）XIIIA：Ag为2．1％、FXIIIS：Ag为2．8％。t－PA：A及PAI：A未测出。下层液中凝血因子无特异性表达。结论：单核细胞上清液（含胞膜及胞浆）有凝血因子活性。
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| 关 键 词： | 单核细胞 血液凝固 纤维蛋白溶解 |
Study on the coagulant activity of human blood monocytes |
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| Jia Xiaomei,Zheng Huan,Wang Jing,et al.. Study on the coagulant activity of human blood monocytes[J]. Chinese Journal of Hematology, 1997, 18(9): 474-476 |
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| Authors: | Jia Xiaomei Zheng Huan Wang Jing et al. |
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| Affiliation: | Institute of Hematology, Beijing People's Hospital, Beijing Medical University, Beijing 100044. |
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| Abstract: | OBJECTIVE: To investigate the coagulant and fibrinolytic activities of human blood monocytes. METHODS: Cultured monocytes (2. 8 x 10(6) cells) were refrigerated at -20 degrees C and then thawed and centrifuged. Blood coagulant factors activities in the upper and lower layer of the supernatant were assayed and compared with that in the supernatant of white blood cells (containing 2. 8 x 10(6) cells). RESULTS: There were factors II , VII, IX, X, V, and tissue factor activities in the supernatant, X III A : Ag was 2.1%, X III S : Ag : 2.8%, while t-PA : A and PAI : A were undetectable. In the lower layer there was no specific expression of blood coagulant factors. CONCLUSION: There were coagulant activities in the supernatant containing the membrane and cytoplasma of monocytes. |
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| Keywords: | Monocytes Blood coagulation Fibrinolysis |
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