原发性高血压患者血浆ADMA浓度外周循环内皮祖细胞数量的变化

目的探讨原发性高血压（EH）患者血浆非对称性二甲基精氨酸（ADMA）浓度、外周循环内皮祖细胞（EPCs）数量的变化及两者之间的关系。方法选取停止服用降压药至少2周以上并排除冠状动脉粥样硬化性心脏病、糖尿病的原发性高血压患者46例，对照组25例为健康体检者。采用反相高效液相色谱法（RP—HPLC）测定血浆ADMA含量，流式细胞术分析外周循环EPCs。结果EH组血浆ADMA浓度[（0.363±0．029）μg/ml]显著高于对照组[（0．319±0．022）μg／ml]（P〈0．01）。EH组外周循环EPCs数量[（0．368±0．121）％]显著低于对照组[（0．588±0．139）％]（P〈0．01）。血浆ADMA浓度与外周循环EPCs数量呈负相关关系（r=-0．706，P〈0．01）。结论EH患者血浆ADMA浓度升高，外周循环EPCs数量减少，两者之间呈负相关关系。EH患者循环EPCs数量下降部分原因可能是由于血浆ADMA水平升高所致。

Study on the change of concentration of Plasma asymmetric dimethylarginine(ADMA)and number of circulating endothelial progenitor cells(EPCs)in essential hypertensive patients

Objective To investigate the change of concentration of plasma asymmetric dimethylarginine （ADMA） and number of circulating endothelial progenitor cells（EPCs） in essential hypertensive patients（EH）. Methods 46 EH patients and 25 healthy controls were recruited. Plasma ADMA were measured by reversed - phase high performance liquid chromatogram （ RP - HPLC ）. Circulating EPCs was determinated by flow cytometry assay. Results The concentration of plasma ADMA in EH patients was higher than that in control [ （0. 363 ± 0. 029 ）μg/ml vs （0. 319 ± 0. 022 ） μg/ml, P 〈 0. 01 ] The number of circulating EPCs in EH patients was lower than that in control [ （0. 368± 0. 121 ） % vs （0. 588± 0. 139 ） %, P 〈 0. 01 ]. The concentration of plasma ADMA was correlated inversely with the number of circulating EPCs in EH group （ r = - 0. 706, P 〈 0. 01 ）. Conclusions The plasma ADMA concentration is increased and the number of circulating EPCs is decreased in EH patients. The concentration of plasma ADMA is correlated inversely with the number of circulating EPCs. The number of circulating EPCs is partly decreased because of the elevation of the plasma ADMA concentration.