孕酮调节复发性流产患者淋巴细胞产生Th1、Th2因子及机制

目的探讨孕酮（P）对不明原因复发性自然流产（URSA）患者淋巴细胞产生Th1、Th2因子的调节作用及其机制。方法提取URSA患者的外周血单个核细胞（PBMC）,在植物血凝素（PHA）刺激下,分孕酮组和对照组培养后,采用ELISA法检测细胞培养上清中IL-12、IL-4的浓度,提取细胞全蛋白,用Western Blotting法检测过氧化物酶体增值物激活受体γ（PPAR-γ）的表达。结果孕酮组IL-12水平显著低于对照组（P〈0.01）,IL-4显著高于对照组（P〈0.05）。孕酮组PPAR-γ的表达显著高于对照组（P〈0.05）。结论孕酮能调节URSA妇女PBMC产生的Th1、Th2细胞因子,PPAR-γ可能参与了细胞因子表达的调控,PPAR-γ是重要的免疫调节因子。

The role and mechanism of progesterone in modulating Th1,Th2 cytokine of lymphocyte from women with recurrent abortion

Objective：To investigate the role and mechanism of progesterone in modulating Th1,Th2 cytokine production of lymphocytes from women with unexplained recurrent spontaneous abortion（URSA）.Methods：Peripheral blood mononuclear cells（PBMC）were separated from women with URSA.Both progesterone group and control group were cultured under the stimulating of phytohemagglutinin（PHA）.Culture supernatants were assayed for IL-12,IL-4 by ELISA.The remained cells were extracted for the whole protein to measure the expression of PPAR-γ by Western Blotting.Results：expression of IL-12 in the progesterone group was significant lower than the control（P〈0.01）,while IL-4 in the progesterone group was significant higher than the control（P〈0.05）.The expression of PPAR-γ in the progesterone group were higher than in the control group（P〈0.05）.Conclusion：progesterone can modulate Th1、Th2 cytokine production of PBMC of URSA women,and PPAR-γ may participate in such process,PPAR-γ may be an important immune-mediator.