Affiliation: | aMolecular Parasitology Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran bHepatitis and AIDS Department, Pasteur Institute of Iran, Tehran 1316943551, Iran cImmunology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran dLaboratoire Adaptation et Pathogénie des Microorganismes, CNRS UMR 5163, Joseph Fourier University (Grenoble I), BP 170, 38042 Grenoble Cedex 09, France eParasitology and Mycology Laboratory, Grenoble Teaching Hospital A Michallon, BP 217, 38043 Grenoble Cedex 09, France |
Abstract: | Indirect immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays (ELISAs) with a recombinant GRA6 protein of Toxoplasma gondii were developed and evaluated for accurate diagnosis of recently acquired infection in pregnant women. According to the results from Toxoplasma serodiagnostic tests, women were classified into 3 groups representing acute (group I), chronic (group II), or no Toxoplasma infection (group III). To discriminate group I from group II sera, the GRA6–IgG-ELISA reached sensitivity and specificity of 87.5% and 94.1%, respectively. Although 22 (91.7%) of 24 group I sera were positive by the GRA6–IgM-ELISA, only 1 (2.9%) of 34 group II sera scored positive. The GRA6–IgM-ELISA displayed a meaningful correlation with Vidas Toxo IgM and exhibited higher specificity (97.1%) than Euroimmun IgM ELISA (88.2%) (Euroimmun, Lübeck, Germany) for detection of recent infection. These results demonstrate that IgG and IgM ELISA with rGRA6 are useful to identify and discriminate recent from past Toxoplasma infection in pregnant women. |