不同剂量X射线诱导小鼠腹腔巨噬细胞IL-18表达的量效关系

目的：观察X射线照射对小鼠腹腔巨噬细胞IL-18表达的影响。方法：昆明种小白鼠80只，随机分为10组（每组8只），即假照组、 4个单次低剂量X射线照射组（0.05、0.075、0.1 和0.2 Gy）和5个单次高剂量X射线照射组（0.5、1.0、2.0、4.0及6.0 Gy），X 线全身照射（WBI）后16 h处死小鼠，取腹腔巨噬细胞，用RPMI 1640培养基，在37℃、5％ CO₂培养箱中培养72 h，留取上清液，采用ELISA法检测小鼠腹腔巨噬细胞IL-18的分泌量。结果：0.05 Gy X射线WBI后，IL-18的表达有所增高，检测值为(59.53±3.68)ng•L^-1，与假照组(51.29 ±3.26)ng•L^-1比较差异无显著性（P＞0.05）；0.075 Gy X射线WBI后，IL-18的表达量增高至(122.78±8.07)ng•L^-1，与假照组比较差异有显著性(P<0.01)；随着X射线辐射剂量增加至0.1、0.2 、0.5和1.0 Gy，IL-18的表达量增高至(135.32±5.27)、(149.40±16.54)、(200.42±15.42)及(347.59 ±17.88)ng•L^-1，1.0 Gy照射达到峰值，随后IL-18表达呈下降趋势；2.0、4.0及6.0 Gy WBI后，IL-18的表达量分别为(229.49±14.68)、(253.79±6.69)及(223.32 ±20.85)ng•L^-1，均高于假照组(P<0.01)。结论：高、低剂量X射线照射均能诱导小鼠腹腔巨噬细胞IL-18的表达。

Dose-effect relationship between X-rays in various doses and IL-18 expression in mouse macrophages

Objective To observe the effect of X-rays irradiation on IL-18 expression in mouse peritoneal macrophages. Methods Eiyghty Kunming white mice were divided into 10 groups( eight each group) : sham- irradiation , four groups of low-dose irradiation (0.05,0.075, 0.1 and 0.2 Gy), and five groups of high-dose irradiation (0.5,1.0,2.0,4.0 and 6.0 Gy). The mice were killed 16 h after whole body irradiation(WBI), with X-rays then the peritoneal macrophages were obtained and cultivated with culture medium of RPMI 1640 in culture box with 37℃,5% CO_2 for 72 h. The supernate was gathered to detect the IL-18 expression level in macrophages by ELISA. Results The IL-18 expresson level in sham-irradiation group was (51.29±3.26 )ng·L~ -1 , IL-18 expression was enhanced after 0.05 Gy WBI, but there was no significant difference (P>0.05). The expression level was increased to (122.78±8.07) ng·L~ -1 after 0.075 Gy WBI, and there was significant difference compared with sham-irridiation group(P<0.01). IL-18 expression was increased gradually with the enhancement of X-rays irradiated doses (0.1, 0.2,0.5 and 1.0 Gy), and the values were (135.32±5.27),(149.40±16.54), (200.42±15.42)and (347.59 ±17.88)ng·L~ -1 , respectively. It peaked after 1.0 Gy irradiation, then it decreased after 2.0,4.0 and 6.0 Gy irradiation, and the values were (229.49±14.68),(253.79±6.69) and (223.32±20.85 )ng·L~ -1 , respectively , but they were higher than that in sham-irradiation group(P<0.01). Conclusion Both high-dose and low-dose X-rays irradiation can stimulate IL-18 expression in mouse peritoneal macrophages .