| 多药耐药相关蛋白1与人肝细胞耐砷性关系的研究 |
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| 引用本文： | 申旭波,周远忠,姜慧,贾飞飞,熊云刚,邹焰.多药耐药相关蛋白1与人肝细胞耐砷性关系的研究[J].中国地方病防治杂志,2009(2):81-83. |
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| 作者姓名： | 申旭波 周远忠 姜慧 贾飞飞 熊云刚 邹焰 |
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| 作者单位： | 贵州省遵义医学院预防医学教研室; |
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| 基金项目： | 贵州省科技厅项目编号:C-271(2005)2059 |
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| 摘 要： | 目的探讨多药耐药相关蛋白1（MRP1）与人肝细胞（L-02）耐砷性之间的关系。方法采用人肝细胞L-02细胞株，噻唑兰法（MTT）进行24h急性NaAsO2毒性试验，选择细胞生存率在90％～95％时的NaAsO2浓度为诱导浓度，同时设1组不加砷诱导的L-02细胞作为同步对照。置于细胞培养箱中37℃，5％CO2常规培养6周，定期换液传代，每周用MTT法计算半数致死浓度（LC50）及细胞的生存率；6周后，将加砷诱导的L-02肝细胞与同步对照细胞在培养基中培养至细胞80％融合，然后进行24h急性砷毒性试验（NaAsO2终浓度为0、2．5、5．0、10μmol／L）；采用real-time PCR检测细胞内MRP1mRNA的表达情况；MTT法检测细胞的存活率；石墨炉原子吸收光谱法检测各组细胞内总砷浓度。结果实验组细胞MRP1mRNA的表达明显高于对照组（P〈0．05）；24h急性砷毒性试验中，实验组不同砷浓度下细胞生存率和LC50明显高于对照组（均P〈0．001）；实验组细胞内总砷浓度明显较同步对照组低（P〈0．001）。结论L-02肝细胞具有可诱导的对砷的耐受性，其耐砷性与MRP1高表达有关。
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| 关 键 词： | 多药耐药相关蛋白1 人肝细胞(L-02) 耐受性 亚砷酸钠 |
Overexpression of MRP1 Is Associated with Acquired Tolerance To Arsenic In L-02 Cell |
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| SHEN Xu-bo,ZHOU Yuan-zhong,JIANG Hui,et al.Overexpression of MRP1 Is Associated with Acquired Tolerance To Arsenic In L-02 Cell[J].Chinese Journal of Control of Endemic Disenaces,2009(2):81-83. |
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| Authors: | SHEN Xu-bo ZHOU Yuan-zhong JIANG Hui |
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| Institution: | Department of Preventive Medicine;Zunyi Medical College;Zunyi 563000 |
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| Abstract: | Objective To study the relativity of Multidrug resistance associated protein 1(MRP1) gene expression and Acquired tolerance to arsenic in human hepatocyte cell line(L-02).Methods Human hepatocyte cell line(L-02) were exposed to low-level arsenite for 6 weeks,3-4,5-dimethy Ithiazol-2-yl]-2,5-diphenyl tetrzolium(MTT) was used to detect the survival rate and LC50 of arsenic-exposed cells and the control which can reflect the change of arsenic tolerance.After 24 h cytotoxicity test,we chose the initial dose un... |
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| Keywords: | Multidrug resistance associated protein 1 Human hepatocyte cell Tolerance Sodium arsenite |
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