抗线粒体-M2抗体在原发性胆汁性肝硬化的意义

目的探讨抗线粒体抗体-M2亚型(AMM-M2)对原发性胆汁性肝硬化(PBC)的意义.方法应用酶免疫斑点法(DIBA)检测抗AMA-M2抗体163例,M2抗原包被区出现深蓝或紫色条带为阳性,无颜照色为阴性.结果PBC患者39例,AMA-M2抗体38例阳性,阳性率97.4%;自身免疫性肝炎患者16例,AMA-M2抗体1例阳性,阳性率为6.3%;慢性乙型肝炎、系统性红斑狼疮和正常对照组均阴性.PBC患者AMA-M2抗体阳性率明显高于其它组(P＜0.01).该法灵敏度为97.4%,特异性为98.9%.结论DI-BA检测AMA-M2抗体灵敏度高,特异性强,可作为PBC重要的血清学指标,同时结合临床症状、生化检查、肝组织活检等提高PBC的诊断率.

The Significance of Antimitochondria Antibody-M2 Subtype (AMA-M2) to Primary Biliary Cirrhosis(PBC)

Objective To study diagnosis significance of antimitochondria antibody-M 2 subtype (AMA-M 2) to primary biliary cirrhosis(PBC).Methods Using DIBA to detect AMA-M 2 of 163 cases.It was positive when the coating area of M 2 antigen showed blue or purple strap and negative with no color.Results 39 cases were the patients with PBC,and 38 cases were the patients with positive AMA-M 2.The positive rate was 97.4%.16 cases were the patients with autoimmune hepatitis and only one case was the patient with positive AMA-M 2 .The positive rate was 6.3%.Chronic Hepatitis B group,systemic lupus erythematosus(SLE) group and normal matched contol group were all negative AMA-M 2.The positive rate of AMA-M 2 of PBC patients was much higher than that of other groups (P<0.01) .The susceptibility of this method was 97.4%.The specificity was 98.9%.Conclusion The method of detecting AMA-M 2 by DIBA has higher susceptibility and better specificity.So AMA-M 2 can be an important serology mark.Positive AMA-M 2 with clinical symptom,biochemistry examination,liver biopsy can enhance the diagnosis rate of PBC.