| 甲型H1N1流感病毒长春株的分离鉴定及其分子进化分析 |
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| 引用本文： | 朱晓文,夏咸柱,高玉伟,邬红全,刘红侠,苗志刚,井玉香,李志刚,胡广祥,张忠湛,姜秋杰,胡桂学. 甲型H1N1流感病毒长春株的分离鉴定及其分子进化分析[J]. 中国病原生物学杂志, 2010, 0(12) |
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| 作者姓名： | 朱晓文 夏咸柱 高玉伟 邬红全 刘红侠 苗志刚 井玉香 李志刚 胡广祥 张忠湛 姜秋杰 胡桂学 |
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| 作者单位： | 吉林农业大学动物科技学院;中国人民解放军军事医学科学院军事兽医研究所;吉林省人兽共患病预防与控制重点实验室;吉林省洮南市动物疫病预防控制中心;吉林省动物疫病预防控制中心; |
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| 基金项目： | 国家重点基础研究发展计划项目(No.2011CB505002) |
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| 摘 要： | 目的分离目前流行的甲型H1N1流感病毒,对其进行基因序列测定和遗传变异分析,为研究病毒进化、致病性、流行规律及防治提供科学依据。方法采用MDCK细胞、SPF鸡胚和RT-PCR方法对甲型H1N1流感病毒进行分离、鉴定,并进行核苷酸序列测定和分子进化分析。结果从疑似甲型H1N1流感临床标本中分离得到1株甲型H1N1流感病毒,命名为长春株(A/Changchun/01/2009(H1N1)),基因序列及分子进化分析显示该毒株与2009年大流行的甲型H1N1毒株高度同源,属于流行分支2。相对流行分支1(北美流行株A/California/07/2009(H1N1)),PB2、HA、NA分别出现2个、7个和4个氨基酸的差异。结论甲型H1N1流感病毒长春株与2009年大流行的甲型H1N1毒株高度同源,属于流行分支2,与流行分支1相比存在变异。
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| 关 键 词： | 甲型H1N1流感病毒 分离鉴定 序列分析 |
Identification and molecular evolutionary analysis of influenza A H1N1 virus isolated from Changchun |
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| Affiliation: | ZHU Xiao-wen1,2,3,XIA Xian-zhu2,GAO Yu-wei2,WU Hong-quan4,LIU Hong-xia4,MIAO Zhi-gang4,JING Yu-xiang4,LI Zhi-gang4,HU Guang-xiang4,ZHANG Zhong-zhan5,JIANG Qiu-jie5,HU Gui-xue1 (1.Jilin Agricultural University,Changchun 130118,China,2.Institute of Military Veterinary Medicine,Academy of Military Medical Sciences,3.Key Laboratory of Jilin Province for Zoonosis Prevention and Control,4.Jilin Province Taonan Center for Animal Disease Control and Prevention,5.Jilin Provincial Center for Animal Disease Contro... |
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| Abstract: | Objective To ascertain the genetic characteristics of the influenza A H1N1 virus,strains of influenza A H1N1 virus currently prevalent in Changchun were isolated and identified and their genome sequences were analyzed.Methods RT-PCR was used to detect the virus.MDCK cell lines and SPF eggs were used for virus isolation.Molecular evolutionary analysis was done by sequencing analysis with the isolated gene RT-PCR products.Results One influenza A H1N1 virus strain was isolated from throat swab specimens,and mo... |
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| Keywords: | Influenza A H1N1 virus isolation and identification sequence analysis |
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