| 2型糖尿病患者维医分型及其口腔菌群多样性研究 |
| |
| 引用本文： | 舒方义,哈木拉提·吾甫尔,吐尔洪·吐送,阿不力克木·吐尔逊,艾比拜·玉素甫,德力夏提·依米提. 2型糖尿病患者维医分型及其口腔菌群多样性研究[J]. 中国病原生物学杂志, 2010, 0(5) |
| |
| 作者姓名： | 舒方义 哈木拉提·吾甫尔 吐尔洪·吐送 阿不力克木·吐尔逊 艾比拜·玉素甫 德力夏提·依米提 |
| |
| 作者单位： | 新疆医科大学病原生物学教研室;新疆医科大学维吾尔医学系;新疆医科大学第一附属医院内分泌科; |
| |
| 基金项目： | 国家自然科学基金资助项目(No.30860385) |
| |
| 摘 要： | 目的了解维吾尔医学热性糖尿病和寒性糖尿病人口腔菌群结构的多样性及其与健康个体的差别。方法对26例2型糖尿病患者进行维吾尔医学分型,其中15例为热性糖尿病患者,11例为寒性糖尿病患者。采集26例糖尿病患者和14例健康对照者的口腔细菌样品,提取细菌总DNA,PCR扩增16S rDNA V3可变区,产物经DGGE后借助Quantity One-4.6.8软件计数指纹图谱中每个样本的条带数,进行多样性分析。结果 3组个体的DGGE指纹图谱DNA条带数分别为21.0±3.7、21.7±3.3和20.4±2.7,差异无统计学意义(P=0.36)。结论热性糖尿病组和寒性糖尿病组患者口腔菌群与健康对照组具有同样程度的多样性。
|
| 关 键 词： | 2型糖尿病 维吾尔医学 口腔菌群 16S rDNA 变性梯度凝胶电泳 |
Typing of patients with type-2 diabetes mellitus according to traditional Uighur medicine and a study of the diversity of their oral flora |
| |
| Affiliation: | SHU Fang-yi1,Halmurat·UPUR2,Turghun2,Abulikemu·TUERXUN3,Aibibai·YUSUFU3,Delixiati·YIMITI1 (1.Department of Pathogenic Biology,Xinjiang Medical University,Urumqi 830011,China,2. Department of Uighur Medicine,3. Department of Endocrinology,First Affiliated Hospital of Xinjiang Medical University) |
| |
| Abstract: | Objective To compare the diversity of oral flora in healthy individuals and patients with hot diabetes mellitus and cold diabetes mellitus according to traditional Uighur medicine. Methods In accordance with traditional Uighur medicine,26 patients with type-2 diabetes mellitus (T2DM) were divided into two groups. There were 15 patients in the hot diabetes mellitus group and 11 in the cold diabetes mellitus group. Bacteria samples were collected from these 26 patients and 14 healthy individuals,which served ... |
| |
| Keywords: | T2DM traditional Uighur medicine oral floras 16S rDNA denaturing gradient gel electrophoresis |
| 本文献已被 CNKI 等数据库收录！ |
|
