The complete nucleotide sequence and genome organization of tomato chlorosis virus |
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Authors: | W M Wintermantel G C Wisler A G Anchieta H-Y Liu A V Karasev I E Tzanetakis |
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Institution: | (1) USDA-ARS, Salinas, California, U.S.A.;(2) Department of Plant Pathology, University of Florida, Gainesville, Florida, U.S.A.;(3) Department of Microbiology and Immunology, Thomas Jefferson University, Doylestown, Pennsylvania, U.S.A.;(4) Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, U.S.A. |
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Abstract: | Summary. The crinivirus tomato chlorosis virus (ToCV) was discovered initially in diseased tomato and has since been identified as
a serious problem for tomato production in many parts of the world, particularly in the United States, Europe and Southeast
Asia. The complete nucleotide sequence of ToCV was determined and compared with related crinivirus species. RNA 1 is organized
into four open reading frames (ORFs), and encodes proteins involved in replication, based on homology to other viral replication
factors. RNA 2 is composed of nine ORFs including genes that encode a HSP70 homolog and two proteins involved in encapsidation
of viral RNA, referred to as the coat protein and minor coat protein. Sequence homology between ToCV and other criniviruses
varies throughout the viral genome. The minor coat protein (CPm) of ToCV, which forms part of the “rattlesnake tail” of virions
and may be involved in determining the unique, broad vector transmissibility of ToCV, is larger than the CPm of lettuce infectious
yellows virus (LIYV) by 217 amino acids. Among sequenced criniviruses, considerable variability exists in the size of some
viral proteins. Analysis of these differences with respect to biological function may provide insight into the role crinivirus
proteins play in virus infection and transmission. |
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