不同软性人工晶状体表面细胞粘附的实验研究

目的通过体外研究比较不同软性材料人工晶状体表面的细胞黏附情况。方法选择3类(每类各20枚)人工晶状体,包括亲水性丙烯酸酯、疏水性丙烯酸酯、硅凝胶人工晶状体。每枚人工晶状体在血小板、中性粒细胞、鼠腹腔巨噬细胞、人结膜囊成纤维细胞等细胞悬液中放置后,在倒置相差显微镜下观察其表面4个象限细胞黏附情况并摄像,计数视野内的细胞数,每个标本记录8个数据,取平均值。并在扫描电镜下观察人工晶状体表面细胞的分布和形态。结果血小板和中性粒细胞在亲水性丙烯酸人工晶状体表面粘附的最少,其明显少于疏水性丙烯酸和硅凝胶人工晶状体;小鼠腹腔巨噬细胞在亲水性丙烯酸人工晶状体表面的粘附少于疏水性丙烯酸人工晶状体,而与硅凝胶人工晶状体无显著性差异;人结膜囊成纤维细胞在疏水性丙烯酸人工晶状体表面生长最密集,亲水性丙烯酸人工晶状体表面的成纤维细胞粘附较少,但形态健康。结论不同软性材料的人工晶状体表面细胞黏附情况各有不同。

The Study of Cellular Adherence to the Surface of Different Foldable Intraocular Lenses

Objective To compare the adherence of cells to different soft intraocular lenses（IOLs） in vitro.Methods Three types（20 per styles） of IOLs made of different biomaterials were studied,including hydrophilic acrylic,hydrophobic acrylic,and silicone lenses.Each lens was immersed in cell suspension of platelets,granulocytes,macrophages of mouse abdomen,fibroblast cells of conjunctiva sac and then photographed in balanced salt solution by phase contrast microscope.The distribution and morphology of cells on the surface of intraocular lenses was also observed under scanning electron microscopy.Results In vitro,the platelets and granulocytes on hydrophilic acrylic IOLs were less than that of hydrophobic acrylic IOLs and silicone IOLs which had significant differences.The adhesion of macrophages of mouse on hydrophilic acrylic IOLs was significantly lower than hydrophobic IOLs and similar to silicon IOLs.The hydrophlic acrylic was better than the silicon and inferior to the hydrophobic acrylic.Conclusion Different intraocular lenses show different amounts and distributions of cells on the surface.