Cullin1蛋白在胃癌中的表达及临床意义

目的:研究Cullin1在胃癌细胞及胃癌组织中的表达,并分析Cullin1、临床分期与患者生存率之间的相关性.方法:采用Western blot法检测SGC-7901、BGC-823胃癌细胞与正常胃黏膜上皮细胞GES-1中、胃癌组织与癌旁组织中Cullin1蛋白表达差异.我们利用已经构建的胃癌数据库,采用免疫组织化学法检测792例胃癌组织中Cullin1表达.结果:我们研究发现,SGC-7901、BGC-823胃癌细胞中Cullin1表达水平均高于胃上皮细胞株GES-1(P<0.01).胃癌组织中Cullin1蛋白表达水平均高于癌旁组织(P<0.01).Cullin1过表达与胃癌TNM分期(P=0.011)、浸润深度(P=0.035,T1-T3与T4)及淋巴结转移(P=0.036)显著相关.此外,我们发现Cullin1的高表达与胃癌患者较差的总生存时间及3年生存率明显相关(P=0.042,0.026).Cox回归分析显示,Cullin1表达是胃癌患者3年生存率的一个独立的预后因子(P=0.028).结论:我们的数据表明,Cullin1可作为胃癌淋巴结转移、预后以及潜在治疗的一个重要标志和研究目标.

Clinical signif icance of Cullin 1 protein expression in gastric cancer

AIM:To examine the expression of Cullin 1 protein in gastric cancer cells and tissues and to analyze the correlation between Cullin 1 protein expression and clinicopathologic variables and patient survival.METHODS:Cullin 1 protein expression in BGC-823,SGC-7901,and GES-1 cells was determined by Western blot.In addition,we constructed a tissue microarray that included 792 primary gastric cancer tissues and evaluated Cullin 1 expression in tumor biopsies by immunohistochemistry.RESULTS:Cullin 1 protein expression levels were higher in BGC-823 and SGC-7901 cells than in GES-1 cells(both P < 0.01),and in gastric cancer tissues than in adjacent tissues(P < 0.01).Cullin 1 overexpression was significantly correlated with TNM stage(P = 0.011),depth of invasion(P = 0.035,T1-T3 vs T4),and lymph node metastasis(P = 0.036).Furthermore,we found a strong correlation between high Cullin 1 expression and worse overall and 3-year survival rates in gastric cancer patients(P = 0.042,0.026).Cox regression analysis revealed that Cullin 1 expression was an independent prognostic factor predicting 3-year outcome in patients with gastric cancer(P = 0.028).CONCLUSION:Cullin 1 may be an important marker for lymph node metastasis and prognosis in gastric cancer,representing a potential treatment target for this malignancy.