| 补肾口服液中淫羊藿苷含量的HPLC测定方法研究 |
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| 引用本文： | 陶松,刘玲,刘旭海,许锦珍,张璐.补肾口服液中淫羊藿苷含量的HPLC测定方法研究[J].江西中医学院学报,2012,24(3):48-50. |
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| 作者姓名： | 陶松 刘玲 刘旭海 许锦珍 张璐 |
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| 作者单位： | 1. 江西中医学院 南昌330004
2. 江中药业股份有限公司 南昌 330096 |
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| 摘 要： | 目的:建立补肾口服液中淫羊藿苷的高效液相色谱定量方法.方法:采用ZORBAX SB-C18(4.6mm×250mm,5μm)色谱柱,流动相为甲醇-水(63∶37),流速1.0 mL/min,检测波长为270nm,柱温25℃.结果:淫羊藿苷浓度为0.0224～0.448mg范围内线性关系良好(r=0.9995),平均加样回收率为100.08％,RSD为1.25％(n=6).结论:该方法简便、准确、灵敏度高、专属性强、重现性好,可作为补肾口服液的质量控制方法.
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| 关 键 词： | 补肾口服液 淫羊藿苷 RP-HPLC 含量测定 |
A Methodological Study of the Quantitative Determination of Icariin in Busen Oral Liquid by HPLC |
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| TAO Song
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LIU Ling
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LIU Xu-hai
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XU Jin-zhen
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ZHANG Lu.A Methodological Study of the Quantitative Determination of Icariin in Busen Oral Liquid by HPLC[J].Journal of Jiangxi College of Traditional Chinese Medicine,2012,24(3):48-50. |
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| Authors: | TAO Song LIU Ling LIU Xu-hai XU Jin-zhen ZHANG Lu |
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| Institution: | 1.Jiangxi University of Traditional Chinese Medicine,Nanchang JIANGXI 330004;2.Jiangxi Jiangzhong Pharmaceutical Co.,Ltd.,Nanchang JIANGXI 330096 |
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| Abstract: | Objective: To establish an HPLC method for detemination of Icariin in busen oral liquid.Method: The separation was per formed on a ZORBAX SB-C18 column(4.6mm× 250mm,5μm).The mobile phase was CH3OH-H2O(63:37).gradient elution at flow rate of 1mL/min,the eluent monitored by a DAD detector at 270 nm,The column temperature was 25℃.Result: The linear range of Icariin was 0.0224~0.448mg,r=0.9995。The average recovery was 100.08%,RSD=1.25%。Conclusion: The method is simple,ac curate,sensitive,specific and reproducible.it can be used for quality evaluation and quality control of busen oral liquid. |
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| Keywords: | bushen oral liquid Icariin RP–HPLC Determination |
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