科罗索酸上调Bax表达诱导宫颈癌细胞凋亡

目的探讨科罗索酸对宫颈癌细胞Hela的凋亡诱导作用。方法不同浓度(10～50μmol·L~(-1))科罗索酸处理宫颈癌Hela细胞,MTT法检测细胞活性,SPSS12.0统计软件包计算半数抑制浓度(IC_(50)),流式细胞术检测细胞凋亡,荧光检测法检测凋亡酶caspase-3活性,western blot检测细胞蛋白Bax、Bcl-2表达。结果科罗索酸抑制宫颈癌Hela细胞活性随药物浓度及时间的增加而增高,24、48及72 h的IC_(50)分别为45、34、28μmol·L~(-1)。科罗索酸诱导Hela细胞凋亡率明显高于对照组(P<0.01),凋亡酶活性也明显高于对照组(P<0.01),科罗索酸可上调Bax而不影响Bcl-2的表达。结论科罗索酸可抑制宫颈癌Hela细胞活性,诱导细胞凋亡,其机制可能与上调Bax蛋白表达有关。

Corosolic acid induces apoptosis by up-regulation of Bax in human cervix adenocarcinoma cells

AIM To investigate the effects of corosolic acid on apoptosis of Hela cells.METHODS Hela cells were treated with various concentrations of corosolic acid(10-50μmol·L^-1).The cell viability was determined by MTT assay.IC₅₀ was calculated by SPSS 12.0.The apoptotic cell death was determined by flow cytometry.The activities of caspase-3 were studied using the caspase fluorometric assay kits.The protein expression of Bax and Bcl-2 were determined by western blot analysis.RESULTS Corosolic acid inhibited Hela cells proliferation in a concentration and time-dependent manner.The 50%inhibitory concentration(IC₅₀) was 45,34 and 28μmol·L^-1 after 24,48 and 72 hours incubation,respectively.Apoptotic rate and caspase-3 activities of the corosolic acid group were higher than those of the control group（P < 0.01）.Corosolic acid upregulated the protein expression of Bax,but not Bcl-2.CONCLUSION Corosolic acid could inhibit the activities of Hela cells and induce the apoptosis of cervix adenocarcinoma Hela cells,which might be related to the upregulation of Bax.