肌圆锥内注射透明质酸对幼兔眼眶发育的影响

目的　观察幼兔肌圆锥内注射透明质酸刺激眼眶生长发育的程度及引起眼眶容积的变化。方法　选取３０只新西兰幼兔（１个月龄），随机分为３组，其中Ａ组、Ｂ组各１０只，均摘除左眼球，保留右眼，Ａ组在术后７ｄ，左眼肌圆锥内注入透明质酸２ｍＬ，术后１个月补充注射透明质酸１ｍＬ，Ｂ组不注射药物；对照组（Ｃ组）１０只，不作任何处理。比较术前和术后１个月、６个月各组左眼睑裂长度及眼眶容积差异。结果　术后１个月Ａ组、Ｂ组、Ｃ组兔左眼睑裂长度分别为（１５．１９±０．４０）ｍｍ、（１４．４３±０．３１）ｍｍ、（１５．２８±０．５５）ｍｍ，术后６个月分别为（１７．６４±０．６４）ｍｍ、（１５．７１±０．９４）ｍｍ、（１９．１１±０．８２）ｍｍ。术后１个月Ａ组、Ｂ组、Ｃ组ＣＴ测量兔左眼眶容积分别为（２．３０±０．１４）ｃｍ３、（１．５４±０．１６）ｃｍ３、（２．４０±０．０９）ｃｍ３，术后６个月分别为（４．７０±０．１８）ｃｍ３、（３．１８±０．１７）ｃｍ３、（４．９４±０．２４）ｃｍ３。术后１个月兔左眼睑裂长度及眶容积，Ｂ组与各组间差异均有统计学意义（均为Ｐ＜０．０１），Ａ、Ｃ组间差异均无统计学意义（均为Ｐ＞０．０５）。术后６个月兔左眼睑裂长度及眶容积，组间两两比较差异均有统计学意义（均为Ｐ＜０．０５）。结论　肌圆锥内注射透明质酸较不注射透明质酸兔眼眶发育好，是促使眼睑及眼眶发育的有效方法。

Effects of injecting hyaluronic acid into muscle cone on orbital development of immature rabbits

Objective To observe the orbital growth and changes of orbital volume stimulated by injecting hyaluronic acid into immature rabbits muscle cone. Methods A total of 30 New Zealand immature rabbits with I month old were randomly divided int0 3 groups,10 cases in each group. The left eyes of rabbits in group A and B were enucleated,the right eyes were remained. Seven days later after the surgery, the left eye muscle cones of each rabbits in group A were injected 2 mL hyaluronic acid . and added I mL one month later. Rabbits in group B were not injected drugs, while rabbits in group C were done nothing. The left palpebral fissure length and orbital volumes were measured at preoperative and postoperative I month ,6 months. Results At postoperative I month ,the left palpebral fissure length in group A,B and C were ( 15. 19 +0. 40 ) mm. ( 14. 43 + 0. 31 ) mm. ( 15. 28 + 0. 55 ) mm, respectively, which at postoperative 6 months were ( 17. 64 + 0. 64 ) mm . ( 15. 71 + 0. 94 ) mm , ( 19. 11 + 0. 82 ) mm , respectively. At postoperative I month .the left orbital volumes measured by CT in group A, B and C were ( 2. 30 +0. 14) cm3 , ( 1. 54 + 0. 16 ) cm3 . ( 2. 40 + 0. 09 ) cm3 , respectively , which at postoperative 6 months were ( 4. 70 +0. 18) cm3 . (3. 18 + 0. 17 ) cm3 . (4. 94 + 0. 24 ) cm3 . respectively. The differences in the left palpebral fissure length and orbital volumes at postoperative I month between group B and the other groups were statistically significant ( all P < 0. 01 ) , the difference between group A and C was not statistically significant (P > 0. 05 ) . The differences in the left palpebral fissure length and orbital volumes at postoperative 6 months between each two groups were statistically significant ( all P < 0. 05 ) . Conclusion The orbital growth with hyaluronic acid injection is better than that without injection , and it is a effective way to increase palpebral fissure length and orbital volume.