红细胞生成素对慢性肾衰竭大鼠外周血内皮祖细胞的影响

目的 探讨红细胞生成素（EPO）对慢性肾衰竭大鼠外周血内皮祖细胞（EPC）数量和功能的影响。 方法 采用分阶段5/6肾切除制备大鼠慢性肾衰竭模型。实验动物按数字随机表法分为4组（均n = 7）：假手术组、慢性肾衰竭组（模型组）、30 U/kg EPO干预组（小剂量组）和50 U/kg EPO干预组（大剂量组）。大鼠皮下注射EPO 6周后，取其外周血分离培养EPC，并检测EPC数量及其增殖、黏附和形成血管结构的能力。 结果 与假手术组比较，慢性肾衰竭大鼠外周血EPC数量及其增殖、黏附与形成血管结构的能力均显著下降（均P < 0.05）。应用EPO治疗能显著增加慢性肾衰竭大鼠外周血EPC数量（P < 0.05），改善外周血EPC增殖、黏附及形成血管结构的能力（均P < 0.05），并且呈剂量依赖性。 结论 EPO可改善慢性肾衰竭大鼠外周血EPC的数量和功能。

Effects of erythropoietin on peripheral blood endothelial progenitor cells from rats with chronic kidney disease

Objective To investigate the effects of erythropoietin (EPO) on the number and function of peripheral blood endothelial progenitor cells (EPCs) from rats with chronic renal failure (CRF). Methods The model of chronic renal failure was established by a two-stage 5/6 nephrectomy procedure in rats. Experimental rats were randomly divided into four groups(n=7, respectively): sham operation group, CRF group, CRF rats treated with 30 U/kg EPO (low-dosage group) and with 50 U/kg EPO (high-dosage group). CRF rats were given EPO by hypodermic injection for 6 weeks, then EPCs were isolated by density gradient centrifugation from peripheral blood mononuclear cells. The ability of cell proliferation, adhesion and vasculogenesis in vitro was further observed. Results Compared to sham operation group, the ability of cell proliferation, adhesion and vasculogenesis in vitro in CRF rats was remarkably decreased (P<0.05, respectively). Such ability was promoted significantly in dose-dependent manner by EPO treatment (P<0.05, respectively). Conclusion EPO can improve the number and ability of endothelial progenitor cells from rats with chronic renal failure.