| EGCG对体外培养子宫肌瘤细胞增殖和凋亡的影响 |
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| 引用本文： | 张秋妍,李美蓉. EGCG对体外培养子宫肌瘤细胞增殖和凋亡的影响[J]. 中国医疗前沿, 2011, 6(1): 14-15 |
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| 作者姓名： | 张秋妍 李美蓉 |
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| 作者单位： | 山西医科大学第一临床医学院妇产科; |
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| 摘 要： | 目的研究EGCG对体外培养子宫肌瘤细胞增殖和凋亡的作用,及对p-AKT和PTEN蛋白表达的影响。方法体外培养子宫肌瘤细胞,不同浓度EGCG干预后,应用四甲基偶氮唑蓝（MTT）法检测细胞增生活性;PI染色FCM法分析检测细胞周期变化及凋亡率;免疫细胞化学测定p-AKT和PTEN蛋白表达情况。统计学分析采用方差分析和t检验。结果 EGCG对体外培养子宫肌瘤细胞有增殖抑制作用（P〈0.05）,呈时间、浓度依赖性,以剂量依赖方式诱导子宫肌瘤细胞凋亡（P〈0.05）,上调PTEN表达,下调p-AKT表达。结论 EGCG通过上调PTEN表达、下调p-AKT表达抑制肌瘤细胞的体外增生。
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| 关 键 词： | EGCG 子宫肌瘤 p-AKT PTEN |
Effect of EGCG on the proliferation and apoptosis of human uterine leiomyoma cells in vitro |
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| ZHANG Qiu-yan,LI Mei-rong. Effect of EGCG on the proliferation and apoptosis of human uterine leiomyoma cells in vitro[J]. China Healthcare Innovation, 2011, 6(1): 14-15 |
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| Authors: | ZHANG Qiu-yan LI Mei-rong |
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| Affiliation: | ZHANG Qiu-yan,LI Mei-rong.Department of Obstetrics and Gynecology,First Clinical Medical College,Shanxi Medical University,Taiyuan030001,China |
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| Abstract: | Objective To investigate the expression of p-AKT and PTEN and the suppressive effect of EGCG on the proliferation and apoptosis of human uterine leiomyoma cells in vitro.Methods The proliferation of cells was detected by MTT methods.The distribution of cell cycle and the rate of apoptosis were determined by flow cytometry.The expression of p-AKT and PTEN protein was detected by immunocytochemistry.The statistical analysis of the results was performed with t text and analysis of variance.Results EGCG could i... |
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| Keywords: | EGCG Uterine leiomyoma p-AKT PTEN |
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