PTEN-PKB途径在子宫内膜癌中的作用

目的探讨PTEN、PKB在子宫内膜癌发病过程中的作用。方法应用逆转录聚合酶链反应 (RT-PCR)技术检测55例子宫内膜癌组织,13例子宫内膜非典型增生,11例增殖期内膜中PTEN及PKB mRNA的表达。结果 PTEN mRNA在正常内膜、非典型增生内膜及子宫内膜癌组织间表达阳性率分别为 81．8％、38．5％及36．4％,三者比较有显著性差异(P<0．05),而PKB mRNA在正常内膜、非典型增生及子宫内膜癌中表达阳性率为27．3％、46．2％及61．8％,但无统计学差异(P>0．05);在子宫内膜癌不同临床分期、病理分级之间,PTEN mRNA和PKB mRNA表达量均没有显著差异(P>0．05)。结论 PTEN可能通过PI3K- PKB途径导致子宫内膜癌的发生。

Role of PI3K-PKB Pathway in Endometrial Carcinoma

Objective To investigate the role of PTEN and PKB in the occurenc of endometrial carcin-oma.Methods Dectect the expression of PTEN and PKB mRNA in 52 cases with endometrial carcinoma and 11 cases with endometrial hyperplasia by RT-PCR, take 19 cases with normal endometrium as control. Results Positive rate of PTEN mRNA in normal endometrium was 81.8% , and the positive rate in endometrial hyperplasia and carcinoma was 38.5% and 36.4%. The difference between the three groups was remarkable(p<0.05). Positive rate of PKB mRNA in normal endometrium , endometrial hyperplasia and carcinoma was27.3%, 46.2% and 61.8%.There was no significant difference between the three groups (p>0.05).The positive rate of PTEN and PKB expression in different clinical stage, pathological grade and muscular invasion of endometrial carcinoma was of no difference. Conclusion PTEN can regulate PI3K-PKB pathway,leading to the onset of endometrial carcinoma.