平调饮对小鼠H_(22)肝癌模型凋亡基因的影响

目的:研究平调饮对H22荷瘤小鼠Bcl-2、P53和Bax凋亡基因的影响,从而探讨平调饮治疗原发性肝癌的作用机制。方法:昆明种雄性小鼠80只,接种H22肝癌细胞后,随机分为生理盐水组、中药组、西药组及中西医结合四组,每组20只。造模第2d灌胃给药,连续7d,第8d脱颈处死。将肿瘤取出制作小鼠肿瘤细胞切片,进行HE染色,观察病理变化;用免疫组织化学方法对肿瘤中的Bcl-2、P53和Bax蛋白进行染色。用SPSS12.0软件统计结果。结果:造模后西药组小鼠状态较差,其体重明显低于中药组及荷瘤组(P0.01)。HE染色中药组的肿瘤细胞体积缩小,核固缩,核分裂相明显少于模型组,肿瘤组织可见片状坏死区。中药组和中西医结合组的Bcl-2和P53蛋白表达率明显降低,Bax蛋白的表达率则明显增高,与对照组比较有显著性差异(P0.01)。结论:平调饮对肝癌细胞具有抑制作用,其机制可能为通过对肿瘤细胞内P53、Bcl-2、Bax蛋白表达的改变,使肝癌细胞发生细胞凋亡,从而抑制肿瘤生长。

Study on the Effects of Pingtiaoyin on Apoptotic Genes of Hepatoma Mice Models

Objective：To investigate the anti-tumor effect of Pingtiaoyin to apoptotic gene bcl -2、P 53 and bax in mice H22 and its mechanism on programmed cell death （PCD）,and discuss the related mechanism to primary hepatic carcinoma （PHC）. Methods：After 80 male KM mice were injected H22 tumor cells,they were divided into 4 groups：the model group（A）,the chinese medicine group（B）,the western medicine group（C）,the combining traditional Chinese and western medicine group （ D）. From the next day after injected,the groups were repectively fed by medicine,once daily for 7 days. The mice were killed in the eighth day and tumors were excavated and weighted. The H22 tumor from mice was collected and the anti-tumor rate calculated; make the slide of the H22 tumor from the mice and to observe by HE technique;noted the pathological changes;the expression of Bax ,Bcl -2 and P 53 protein in the tumor tissue was studied by immuno histo-chemical method and studied the result by spss12. 0. RESULTS：After the animal experiment,the weight of the mice in C group was lighter than that of the other groups（P 0. 05）. The tumor cells of group B smaller in size,karyopyknosis is significantly less than the model set,tumor tissue was found sheets of necrotic area. The expression frequency of Bcl -2 and P 53 protein of B、D group were lower than A group（P 0. 05）,that of Bax protein of B、D group were significant higher than it,significant difference was found between these groups （P 0. 05）. CONCLUSION：Pingtiaoyin can inhibit the Hepatoma cell,its mechanism may be induced apop-tosis of Hepatoma cell by changing the expression of Bax ,Bcl -2 and P 53 protein in the tumor tissue ,there-by inhibiting tumor growth.