鱼油对急性坏死性胰腺炎大鼠胃肠功能的影响

目的 探讨鱼油对急性坏死性胰腺炎胃肠道功能的影响及可能机制.方法 SD大鼠被分为三组:急性坏死性胰腺炎组(A组,n=8)、急性坏死性胰腺炎鱼油干预组(F组,n=8)和对照组(C组,n=8).观察5 d后各组大鼠胰腺组织病理变化.同时检测各组大鼠胃肠道食物残渣量及血浆超氧化物歧化酶SOD、谷胱甘肽过氧化物酶(GSH-Px)活力、D乳酸浓度和淀粉酶浓度.结果 病理学检查提示A组和F组均表现为严重的胰腺坏死,但F组的炎细胞浸润程度显著低于A组(P<0.01).与C组比较,A组胃肠道食物残渣分布出现异常,其D乳酸水平显著升高(P<0.01);F组胃肠道食物残渣分布与C组比较无统计学意义,D乳酸水平显著低于A组(P<0.01).A组GSH-Px、SOD显著低于C组(P<0.01);F组GSH-Px、SOD显著高于A组(P(0.01).结论 ANP时除表现为胰腺局部严重的炎症外,同时还并发存在胃肠道功能障碍及全身抗氧化防御系统损坏(GSH-Px、SOD是体内最重要抗氧化酶);应用鱼油干预可使ANP大鼠胰腺局部炎症减轻、ANP胃肠道功能障碍得以纠正,可能与其影响抗氧化酶系统有关.

Effect of fish oil on gastrointestinal function of rats with acute necrotizing pancreatitis

Objective To investigate the effect of fish oil on the gastrointestinal function of rats with acute necrotizing pancreatitis and explore its mechanism. Methods The male Sprague-Dawley (SD) rats were divided into three groups: ANP group(group A,n=8), ANP fish oil treatment group(group F,n=8) and control group(group C, n = 8). Five days after experiment being started, the grade of the severity of pancreatitis was evaluated by histological analysis,and the amount of food residue in the rat's gastrointestinal tract in each group were observed. Finally, the activity of serum antioxidant enzymes(SOD, GSH-Px), D-lactic acid and the concentration of serum amylase in each groups were measured respectively. Results His-topathological analysis suggested that the infiltration of the inflammatory cells in group F were significantly weaker than in group A (P<0. 01). The distribution of food residue in the rat's gastrointestinal tract sug-gested that fish oil can improve the obstacle of the gastrointestinal motility in ANP rats. The serum level of D-lactic acid was significantly higher in group A than in group F and C (P<0.01). There was no statistical significance between group C and F. Serum glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels in group A were significantly lower than those in group C (P<0.01). Compared with group A, the treatment of fish oil increased these level in group F significantly. Conclusion Fish oil can improve the obstacle of the rat's gastrointestinal motility and harrier function induced by acute necrotizing pancreati-tis. The mechanism might be related to influencing the activity of antioxidant enzyme system.