骨关节炎患者骨桥蛋白、IL-1β表达水平及意义

目的检测骨原发性关节炎（OA）患者骨桥蛋白（OPN）、IL-1β表达水平的变化及意义,并探讨其与骨关节炎的相关性。方法选取2012年1月-2014年1月确诊为原发性OA患者的软骨标本20例作为OA组,以同期因意外受伤截肢的20例正常软骨标本为对照组。采用HE染色法观察组织学变化,免疫组化法检测关节软骨中OPN、IL-1β表达水平,并采用SamplePCI图象分析系统进行半定量分析。结果正常软骨细胞外基质染色均匀,分散于软骨基质之中,细胞核着色清晰均匀,胞核、胞质清晰可辨。随着OA病变程度的加重软骨细胞基质染色不均,甚至出现纤维化,细胞数目逐渐减少。经免疫染色后,OPN、IL-1β吸光值表示OPN、IL-1β表达水平均升高（P〈0.05）。相关分析得出,OPN与IL-1β呈正相关（r=O.899,P〈0.01）。软骨病变程度分级与OPN、IL-1β呈正相关（r=0.909,0.904,P均〈0.01）。结论原发性OA患者软骨组织中OPN、IL-1β表达水平升高,且与病变程度具有密切联系,为OA治疗的靶点提供了依据。

OPN,IL-1 Beta Change and Significance of the Expression Level in Patients of OA

Objective detection of bone in patients with primary osteoarthritis（ OA） osteopontin（ OPN）,IL-1 beta change and significance of the expression level,and explore its correlation with osteoarthritis. Methods Between January 2012 and January 2012 diagnosed with primary cartilage specimens of 20 patients with OA as OA group,with the same period,amputation because of accidental injury,20 specimens of normal cartilage as control group. Using HE staining to observe the histological changes,immunohistochemical method in the detection of articular cartilage OPN expression level,IL-1 beta,and semiquantitative analysis using SamplePCI image analysis system. Results Normal cartilage extracellular matrix dyeing uniformity,scattered in cartilage matrix,uniform color clear,nucleus nuclei and clear cytoplasm. As the degree of OA lesions increase cartilage matrix uneven dyeing,appear even fibrosis,the number of cells reduce gradually. After immune dyeing,OPN,IL-1 beta absorbance value indicates that OPN,IL-1 beta expression levels were elevated（ P〈0. 05）. Correlation analysis,OPN was positively correlated with IL-1 beta（ r = 0. 899,P〈0. 01）. Cartilage lesions in the classification and OPN,IL-1 beta were positively correlated（ r = 0. 909,0. 904,P〈0. 01）. Conclusion OPN in patients with primary OA cartilage,IL-1 beta expression levels,and the degree is closely related with pathological changes,provide the basis for OA treatment targets.