人二倍体细胞株HLF-02的建立及其生物学特性

目的：建立HLF-02细胞株，为老年学，肿瘤学，病毒学，药物学和生物遗传学等研究提供新的实验材料。方法：人二倍体细胞株HLF-02取于肺组织，用胰蛋白酶消化肺组织，可获90％以上具有正常代谢活性的肺细胞，对存活的肺成纤维细胞进行形态学观察、生长特性检查、核型分析、电镜观察和异种移植等。结果：建成的HLF-02细胞株其形态表现、电镜观察、核型分析和异种移植等均符合二倍体细胞特征。经过10个月的体外培养，共传50代，细胞倍增时间75．5h，染色体始终保持二倍体特性，异种移植成瘤试验阴性。无支原体污染。结论：HLF-02是一株人二倍体细胞株，可用于对细胞老年学，肿瘤学，病毒学，药物学和生物遗传学等方面的研究。

Establishment and characteristics of the human diploid cell strain HLF-02]

OBJECTIVE: To establish the HLF-02 and provide a new material to the study of geronics, oncology, virology, pharmacology, and biogenetics. METHODS: The lung tissue was digested with 0.15% trypsin. More than 90% of the dissociated cell exhibited initial viability (trypan blue dye exclusion and the ability of adhesion). The surviving cells were analyzed by morphology, growth curves, karyotype analysis, electron microscopic observation and heterotransplantation. RESULTS: A newly established cell strain, which was designated HLF-02 had been maintained in continuous culture for over 50 generations for 10 months. Morphological observation, electron microscopic observation, karyotype analysis and heterotransplantation showed that it was corresponding to diploid cells. The doubling time of the cells was 75.5 hours. Chromosome analyses showed the human cell strain to be typical diploid appearance, and the tumorigenecity assays of nude mice were negative. CONCLUSION: The HLF-02 is a human dipolid cell strain and can serve the further studies of cell gerontics, oncology, virology, pharmacology, and biogenetics.