益气活血药对血管紧张素Ⅱ诱导的心肌细胞能量与脂质过氧化反应变化的影响

目的观察心肌细胞在血管紧张素Ⅱ(AngⅡ)作用后反映能量的酶与脂质过氧化反应的变化及不同类中药对其的影响.方法用AngⅡ作用于培养的SD大鼠乳鼠心肌细胞,观察作用24、48、72、96 h细胞上清液中乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)的变化及益气、活血、益气活血中药的作用.结果在AngⅡ作用48 h后,细胞上清液中LDH、SDH明显增加(P＜0.01),MDA含量明显升高,SOD活性明显降低(P＜0.01),各中药组LDH、SDH明显降低,且可明显减少MDA含量、升高SOD活性(P＜0.05或P＜0.01).结论AngⅡ作用一定时间后,伴随着能量的变化和引发的脂质过氧化反应,使心肌细胞的功能受到影响,益气、活血、益气活血中药均有保护作用,尤以益气活血药作用明显.

Effects of some Chinese herbs on changes of energy and lipid peroxidation in cardiac cells induced by Angiotensin Ⅱ

Objective To observe the changes of enzyme which reflected the energy and lipid peroxidation of cardiac cells induced by Angiotensin II(AngII),and different effects of some different Chinese herbs with the effects of replenishing qi and activating blood on these indexes.Method AngII was used in the cultured cardiac cells of SD mouse.The changes of contents of L-lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),malondialdehyde(MDA) and activity of superoxide dismutase(SOD) in cell supernate were observed after 24,48,72 and 96 hours.The effects of above Chinese herbs were also observed.Result After 48 hours of AngII was used the contents of LDH,SDH and MDA decreased obviously(P<0.01) in cell supernate.The activity of SOD decreased obviously(P<0.01),while in different groups with different Chinese herbs the contents of LDH,SDH and MDA increased obviously and the activity of SLD increased(P<0.05 or P<0.01).Conclusion The function of cardiac cells was influenced by the changes of energy and lipid peroxidation induced by AngII.The Chinese herbs with the effects of replenishing qi and activating blood can protect the cardiac cells.