去甲斑蝥素致大鼠腹腔炎症与多脏器功能失常综合征

目的：探讨去甲斑蝥素致大鼠失控炎症在多脏器功能失常综合征（ＭＯＤＳ）发病中的作用。方法：将去甲斑蝥素（一种能刺激皮肤发泡以收集巨噬细胞的致炎物质，１５ｍｇ／ｋｇ）注入６０只Ｗｉｓｔａｒ大白鼠腹腔，动态观察其对炎性介质以及器官形态和功能的影响。结果：与对照组比较，实验组动脉血氧分压（ＰａＯ２）和血小板计数明显下降，谷丙转氨酶（ＧＰＴ）和血肌酐（ＳＣｒ）明显升高，１２０小时后上述指标均接近对照组；１２小时和２４小时大鼠死亡率分别为１５％和２０％；肿瘤坏死因子（ＴＮＦ）和磷脂酶Ａ２（ＰＬＡ２）在注射后２４小时和１２小时达最高值；肺、心、肝、肾、肠等器官出现病理改变。结论：去甲斑蝥素可能通过激活巨噬细胞、释放ＴＮＦ等炎性介质诱发全身炎症和ＭＯＤＳ。

A trial to reproduce multiple organ dysfunction syndrome in animals with intraperitoneal injection of cantharidin

Objective:To evaluate the potential role of excessive inflammation in the development of multiple organ dysfunction syndrome(MODS).Methods:In 60 Wistar rats,cantharidin was intraperitoneally injected in a dose of 15 mg/kg(0 5 mg cantharidin in 1 ml of paraffin).Twenty rats in control group received paraffin in the same volume.Functional parameters and pathological examination of vital organs were determined at various intervals (12,24,72 and 120 hours,and tumor necrosis factor(TNF) and phospholipase A 2(PLA 2) levels were also measured.Results:Cantharidin showed injurious effects on organ functions,especially at 24 hours after challenge,as evidenced by marked decrease in (PaO 2) and platelet count,and elevation in glutamic pyruvic transaminase(GPT) and creatinine(Cr)values.Mortality rates at 12 and 24 hours were 15% and 20%,respectively.On the other hand,TNF and PLA 2 levels were significant higher in cantharidintreated at 24 hours and 12 hours.Pathological changes included edema,hemorrhage,and cellular infiltration in the liver,lung,and intestine.Conclusions:The injurious effects of cantharidin on various organs may be related to release of TNF after activation of macrophages.