P选择素单克隆抗体对大鼠肝缺血—再灌注损伤的治疗作用研究

目的：探讨Ｐ选择素单克隆抗体（单抗）对大鼠肝缺血再灌注损伤的保护作用。方法：在肝缺血再灌注大鼠模型上观察了细胞间粘附分子１和Ｐ选择素的变化，并用Ｐ选择素单抗对其进行阻断治疗。结果：缺血再灌注大鼠肝细胞发生水肿及空泡变性，血清天冬氨酸转氨酶和丙氨酸转氨酶水平升高；但再灌注前５分钟经静脉注射Ｐ选择素单抗，则肝组织与正常组相近，肝细胞未见空泡变性，血清酶水平明显减低；酶联免疫吸附试验发现，再灌注后血清ＩＣＡＭ１和血浆Ｐ选择素水平显著升高，经Ｐ选择素单抗处理的大鼠血ＩＣＡＭ１和Ｐ选择素明显下降。结论：ＩＣＡＭ１和Ｐ选择素与缺血再灌注损伤密切相关，Ｐ选择素单抗对肝缺血再灌注损伤具有保护作用。

The significance of intercellular adhesion molecule1 and Pselectin in hepatic ischemiareperfusion injury

Objective:To investigate the potential role of intercellular adhesion molecule1 ( ICAM1) and Pselectin in hepatic ischemiareperfusion injury.Methods:Using a rat model of 60minute liver ischemia followed by reperfusion,animals were divided into 3 groups:the shamoperated controls,ischemic group receiving only normal saline,and treated group receiving Pselectin monoclo nal antibody (Mab) at a dose of 2 mg/kg at 5 minutes before reperfusion.The following parameters were analyzed such as liver injury tests,liver histological examination,serum enzymes levels aspartate aminotransferase (AST),alanine aminotransferase(ALT) ,and the levels of ICAM1 and P selectin .Results:Serum AST and ALT levels were significantly increased during 60minute left lobar ischemia and reperfusion,while the increment was significantly inhibited,and liver pathology observed by light microscopy was remarkably ameliorated by treatment with Pselectin Mab.Also,the elevated levels of ICAM1 and Pselectin were found in animals after hepatic ischemiareperfusion,but they were markedly reduced in treatment group.Conclusions:ICAM1 and Pselectin may contribute to the development of hepatic ischemiareperfusion injury,and the Pselectin Mab has protective effect on acute hepatic damage induced by local ischemiareperfusion injury.