大黄抗内毒素性休克大鼠炎性介质作用的实验研究

目的：研究大黄对内毒素性休克大鼠炎性介质作用的机制。方法：选用大鼠内毒素性休克模型。随机分为６组：单纯手术组、内毒素组、大黄预防用药组（１５０ｍｇ／ｋｇ组和７５０ｍｇ／ｋｇ组）和大黄治疗组（１５０ｍｇ／ｋｇ组和７５０ｍｇ／ｋｇ组）。检测磷脂酶Ａ２（ＰＬＡ２）和血小板活化因子（ＰＡＦ）的活性。结果：内毒素注射前６组大鼠平均动脉压（ＭＡＰ）无显著性差异；注射内毒素后４小时ＭＡＰ明显降低；大黄预防用药组和大黄治疗组ＭＡＰ则与注射内毒素前及单纯手术组比较均无明显变化，并均显著高于内毒素组注射内毒素４小时后。注射内毒素后４小时，血清和小肠组织中ＰＬＡ２活性及ＰＡＦ含量均明显增高；与内毒素组注射内毒素后４小时比较，大黄预防组和治疗组则血清和小肠组织中ＰＬＡ２活性和ＰＡＦ含量显著降低。结论：大黄对内毒素性休克所致炎症反应有明显的预防和治疗作用

The influence of rhubarb on phospholipase A 2 and platelet activation factor release in rats after endotoxic shock

Objective:To study the mechanism underlying rhubarb inflammatory mediator response in endotoxic shock.Methods:Using a rat endotoxic shock model,animals were randomly divided into 6 groups as follows:endotoxic shock group,sham operation group,rhubarb pretreatment groups ( 150 mg/kg and 750 mg/kg),rhubarb treatment groups(150 mg/kg and 750 mg/kg).Phospholipase A 2(PLA 2) and platelet activation factor (PAF) activities were measured in rats.Results:Mean arterial pressure (MAP) was not significantly different in all groups prior to endotoxin administration,but it significantly decreased at 4 hours in endotoxic shock group.In rhubarb pretreatment and treatment groups,changes in MAP were not markedly different from sham operation group or baseline values,however,they were significantly higher compared to endotoxic shock group.Similarly,PLA 2 and PAF activities in serum and intestinal tissues obviously increased at 4 hours after endotoxin injection,and they significantly decreased in rhubarb pretreatment and treatment groups compared to endotoxic group.Conclusions:Rhubarb has prophylactic and treatment effects in inhibiting septic response caused by endotoxin challenge.