丹参对新生期大鼠缺氧缺血性脑损伤神经肽Y1-36和降钙素基因相关肽的影响

目的：观察丹参对新生期大鼠缺氧缺血性脑损伤（HIBI）后不同时间血浆,桥延脑神经肽Y1－36和降钙素基因相关肽免疫活性物质（ir-NPY,ir-CGRP)含量的影响。方法：7日龄大鼠随机分为HIBI组（A组）,HIBI加丹参治疗组（B组）和假手术组（C组）,每组按不同时间各分成4个小组。在新生期大鼠HIBI后即刻及其后每12h向腹腔注射丹参注射液,每次0.5ml。采用放射免疫分析法观察HIBI后即刻,12,24,48h血浆和桥延脑ir-NPY,ir-CGRP含量的变化。结果：HIBI后不同时间血浆ir-NPY,ir-CGRP含量均显著升高;桥延脑ir-CGRP,ir-NPY含量不同程度升高或降低。应用丹参注射液后血浆,桥延脑ir-NPY水平的上升被部分逆转。结论：中枢及外周神经肽Y1－36和降钙素基因相关肽参与HIBI的病理生理过程;丹参部分逆转新生大鼠HIBI后ir-NPY水平的异常升高,可能是丹能促进损伤后脑功能恢复的作用途径之一。

Effect of Salvia Miltiorrhiza on Neuropeptide Y 1-36 and Calcitonin Gene-Related Peptide in Neonatal Rats with Hypoxia-Ischemic Brain Injury

OBJECTIVE: To observe the effects of Salvia miltiorrhiza (SM) on levels of neuropeptide Y1-36 and calcitonin gene-related peptide immune reactive substances (ir-NPY, ir-CGRP) in blood plasma and pons-oblongata after hypoxia-ischemic brain injury (HIBI) in neonatal rats. METHODS: Seven-day old rats were randomized into HIBI group (A), HIBI + SM group (B) and sham operation group(C). And each group was subdivided into 4 subgroups according to the different time after operation. 0.5 ml SM was injected intraperitoneally immediately and every 12 hrs afterwards. Changes of ir-NPY and ir-CGRP levels in plasma and pons-oblongata were observed immediately and 12, 24 and 48 hrs after HIBI by radioimmunoassay. RESULTS: Plasma levels of ir-NPY and ir-CGRP in different times after HIBI were all significantly raised but those in pons-oblongata were either raised or lowered to a certain degree. Part of the elevated ir-NPY could be reversed by SM injection. CONCLUSION: Central and peripheral neuropeptide Y1-36 and calcitonin gene-related peptide take part in the pathophysiological process of HIBI, SM could partially reverse the abnormal post-HIBI elevation of ir-NPY, which may be one of the pathways of SM in promoting recovery of damaged brain function.