骨髓间质细胞对体外培养的缺氧心肌细胞影响的初步研究

目的：探讨缺氧条件下骨髓间质细胞对培养 的心肌细胞凋亡的作用。方法:共培养乳鼠心肌细胞和骨髓间质细胞。 利用厌养罐模拟细胞缺氧，观察细胞形态、检测细胞凋亡亚二倍体、观察细胞脱氧核苷酸断 裂情况及凋亡相关蛋白的表达。 结果:单独培养的骨髓间质细胞对缺 氧不敏感，而单独培养的心肌细胞在缺氧后24 h出现凋亡，表现为明显的凋亡亚二倍体峰和 “DNA ladder”。当两种细胞缺氧共培养24 h后，混合细胞的凋亡亚二倍体峰明显减少，“ DNA ladder”消失，抗凋亡蛋白Bcl-2表达无显著差异而促凋亡蛋白Bax表达降低。 结论:缺氧条件下体外细胞共培养模型表明骨髓间质细胞对心肌细胞的凋亡有一 定的拮抗作用，可能是通过抑制Bax蛋白的表达而实现的。

Effect of bone marrow stromal cells on the anoxia cardiomyocyte s in vitro

AIM:To investigate the influence of bone m arrow stromal cells (BMSCs) on the anoxia cardiomyocyte apoptosis.METHODS:Using the anaerobic culture apparatus,the apoptosis of the cardiomyocytes,the BMSCs alone and co-cultured with each other were detect ed by morphological observation,PI staining flowcytometry,electrophoretic gel mobility analysis of DNA fragmentation.Western blotting was used to detect Bax and Bcl-2 protein expression.RESULTS:Compared with control,the BMSCs were unsensitive to an oxic cultured while the anoxic cardiomyocytes were prone to apoptosis.Apoptosis of cardiomyocytes was increased significantly,detected by PI staining and agar ose gel elestrophoresis showed “DNA ladder”.However,when anoxia cardiomyocyt es co-cultured with BMSCs,apoptosis cells were decreased,“DNA ladder” disapp eared and the expression of protein Bax was also decreased.CONCLUSION:Bone marrow stromal cells prevent the anoxia cardiom yocytes from apoptosis,probably by suppressing the expression of Bax protein.