喜得镇对脑缺血再灌注小鼠的行为及海马兴奋性氨基酸受体表达的影响

目的探讨喜得镇对脑缺血再灌注小鼠的行为及海马神经元兴奋性氨基酸N甲基D天门冬氨酸(NMDA)受体表达的影响。方法36只小鼠被随机分为模型组、喜得镇组(用喜得镇溶液灌胃)及假手术组,采用双侧颈总动脉线结、反复缺血再灌注法制作小鼠模型,用跳台试验和水迷宫试验观测其行为学改变;采用免疫组化技术观测各组小鼠海马神经元NMDA受体表达变化,并进行组间比较。结果喜得镇组小鼠学习、记忆成绩优于模型组(均P<0.01),其海马NMDA受体表达明显增高(P<0.01);与假手术组比较差异无显著性。结论喜得镇可改善脑缺血再灌注小鼠学习、记忆成绩,与其提高海马NMDA受体水平有关。

Effects of Hydergine on expression of N-methyl-D-aspartate receptors in hippocampus and actions of mice with ischemia-reperfusion injury

Objective To study the effects of Hydergine on expression of N-methyl-D-aspartate receptors in hippocampus and actions of mice with ischemia-reperfusion injury.Methods 36 mice were randomly divided into model group,Hydergine group and sham-operated group. The models of ischemia-reperfusion injury were made by knotting bilateral common carotid arteries. The behavioral abnormalities were investigated by step-down test and water maze test, and the expressions of N-methyl-D-aspartate receptors in neurons of hippocampus were observed by immunohistochemistry technique. Results Compared with model group, the performance records of learning and memory in Hydergine group were better (all P<0.01) and the expressions of N-methyl-D-aspartate receptors were also higher (P<0.01). There was no obvious difference of the results between Hydergine group and sham-operated group. Conclusions Hydergine improves learning and memory ability in mice models of ischemia-reperfusion injury. High expression of N-methyl-D-aspartate receptor may be involved in its mechanisms.