天元克痛方对福尔马林模型大鼠痛行为和脊髓背角c-fos表达及P物质含量的影响

目的观察天元克痛方对福尔马林所致疼痛模型大鼠痛反应的影响，并探讨其镇痛作用机理。方法采用疼痛行为分级评分法评价天元克痛方对福尔马林疼痛模型大鼠的镇痛作用，采用免疫组织化学方法结合电子计算机图像分析技术研究天元克痛方对福尔马林疼痛模型大鼠脊髓背角浅层c—fos基因表达及P物质含量的影响。结果天元克痛方可明显抑制福尔马林致痛大鼠的疼痛反应(P<0．05)，抑制由疼痛所引起的脊髓背角浅层c—fos表达增强、SP含量增多的效应(P<0．05)。结论天元克痛方对福尔马林疼痛模型大鼠具有明确的镇痛作用，减少脊髓背角神经元对传入的伤害性刺激的反应，可能是其发挥镇痛作用的途径之一。

Effect of Tianyuan Ketong Recipe on the Pain Behavior, c-fos Expression and P Substance Contents in Dorsal Horn of Spinal Cord in Formalin Induced Pain Model Rats

To observe the effect and mechanism of Tianyuan Ketong recipe (TKR) on the pain reaction in formalin induced pain model rats. MethodsThe analgesic effect of TKR was evaluated using pain behavior graded scoring, the c fos gene expression and P substance contents in superficial lamella of spinal cord dorsal horn in model rats were analyzed by means of immunohistochemical analysis and computer image analysis technique. ResultsTKR could markedly inhibit the pain reaction in model rats (P<0.05), and the pain induced elevation of c fos expression and P substance contents could also be suppressed (P<0 05). ConclusionTKR shows definite analgesic effect on formalin induced pain model rats, the reduction of neuron's reaction in spinal cord dorsal horn to afferent noxious stimulation is possibly one of the pathways for its analgesic effect.