茶多酚对内毒素作用下人牙周膜细胞分泌和表达Toll样受体4的影响

目的:观察茶多酚(tea polyphenol,TP)对内毒素(lipopolysaccharide,LPS)作用下人牙周膜细胞(periodontal ligament cells, PDLCs)分泌和表达Toll样受体4(toll-like receptor 4,TLR4)的影响。方法:体外分离及培养PDLCs,实验组分别为LPS和不同质量浓度的TP的不同组合,对照组为仅含1% FBS的DMEM培养液。培养24 h、48 h和72 h后,通过酶联免疫吸附测定法检测TLR4的分泌量,荧光实时定量PCR法检测TLR4的表达。结果:100 mg/L LPS组PDLCs TLR4分泌量显著高于其它各组(P<0.05),加入TP进行干预后实验组与对照组TLR4的分泌量和表达量无显著差异(P>0.05)。结论:TP对LPS作用下PDLCs TLR4的分泌和表达有一定的抑制作用。

Effect of Tea Polyphenols on Secretion and Expression of Toll Like Receptor 4 of Human Periodontal Ligament Cells Stimulated by Lipopolysaccharide.

Objective: To examine the effect of tea polyphonenol (TP) on secretion and expression of toll like receptor 4(TLR4) of human periodontal ligament cells(PDLCs) stimulated by lippolysaccharide. Methods: We isolated PDLCs externally and cultured them. For TP experimental group we used different combination of LPS and TP different concentration, and for the matched group we used DMEM nutrient solutions of 1% FBS. All groups were cultured by 24 h, 48 h and 72 h. The level of TLR4 was examined by ELISA and real-time PCR. Results: 100 μg·mL^-1 LPS can significantly enhance the secretion and expression of TLR4 (P<0.05) , and TP significantly inhibits the secretion and expression of TLR4. Conclusion: TP can induce the secretion and expression of PDLCs stimulated by LPS.