墨西哥仙人掌多糖对人乳腺癌MCF-7细胞凋亡坏死诱导作用的研究

目的观察墨西哥仙人掌多糖对人乳腺癌MCF-7细胞凋亡及坏死的诱导作用。方法将浓度为20μg／mL的墨西哥仙人掌多糖作用于体外培养的人乳腺癌MCF-7细胞，分别培养24h和48h，然后利用流式细胞仪检测人乳腺癌MCF-7细胞的凋亡及坏死情况。结果不同作用时间的墨西哥仙人掌多糖均能够诱导人乳腺癌MCF-7细胞凋亡，并促进该肿瘤细胞发生坏死，与阴性对照组比较差异显著（P〈0．01），尤以48h作用明显，凋亡及坏死肿瘤细胞占培养细胞总数的84．11％。结论墨西哥仙人掌多糖能够诱导人乳腺癌MCF-7细胞的凋亡及坏死。

Studies on the Effects of Mexican Cactus Polysaccharides in Inducing Apoptosis and Necrosis of Human Breast Cancer MCF-7 Cells

Objective To observe the effects of Mexican cactus polysaccharides in inducing apoptosis and necrosis of human breast cancer MCF-7 cells. Methods The human breast cancer cells cultivated in vitro were treated with 20 μ g/mL of polysaceharides of Mexican cactus. The effects of Mexican cactus polysaccharides in inducing apoptosis and necrosis of human breast cancer MCF-7 cells were determined by flow cytometry. Results Compared with negative control group, polysaccharides of Mexican cactus can significantly lead to the apoptosis and necrosis of human breast cancer MCF-7 cells in both 24 hours group and 48 hours group, especially the 48 hours group which total percentage of apoptosis and necrosis was 84.11% in the cultivated cells. Conclusion Polysaceharides of Mexican cactus can induce the apoptosis and necrosis of human breast cancer MCF-7 cells.