动脉粥样硬化性脑梗死患者血浆同型半胱氨酸水平及亚甲基四氢叶酸还原酶基因多态性

目的 探讨血浆同型半胱氨酸水平、亚甲基四氢叶酸还原酶基因多态性与动脉粥样硬化性脑梗死之间的关系.方法 选择性别、年龄匹配的动脉粥样硬化性脑梗死患者(脑梗死组)68例及对照组50例,采用荧光偏振免疫法测定血浆同型半胱氨酸水平,聚合酶链反应-限制性片长多态性技术检测亚甲基四氢叶酸还原酶基因多态性.结果 脑梗死组TT基因型(36.8%比16.0%)及T等位基因频率(59.6%比38.0%)均显著高于对照组(P<0.05).脑梗死组血浆同型半胱氨酸水平显著高于对照组(P<0.05).脑梗死组和对照组亚甲基四氢叶酸还原酶 677TT纯合子血浆同型半胱氨酸水平均显著高于CT型和CC型者(P<0.05).结论 血浆同型半胱氨酸水平升高是动脉粥样硬化性脑梗死的危险因素.亚甲基四氢叶酸还原酶 C677T基因多态性与血浆同型半胱氨酸水平密切相关,与动脉粥样硬化性脑梗死显著相关.

The Homocysteine Levels and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Atherosclerotic Cerebral Infarction

Aim To investigate the relationship between plasma homocysteine(Hcy),polymorphisms of the methylenetetrahydrofolate reductase(MTHFR) gene and atherosclerotic cerebral infarction. Methods Plasma Hcy,polymorphisms of the MTHFR gene in 68 patients with atherosclerotic cerebral infarction(CI) and 50 controls were measured by fluorescence polarization immunoassay(FPIA) and polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) respectively. Results The frequencies of TT genotype and T allele were significantly higher in CI group than in controls(P><0.05).Plasma Hcy levels was significantly higher in CI group than in controls(P><0.05).The homocysteine concentration was significantly higher in TT genotype than CT and CC genotypes both in CI group and in control group(P><0.05). Conclusion Elevated Hcy levels is a risk factor for atherosclerotic cerebral infarction.MTHFR C677T polymorphism was significantly related to plasma Hcy levels and atherosclerotic cerebral infarction.