丙型肝炎病毒准种复杂性与干扰素疗效关系的初步探讨

目的：探讨丙型肝炎病毒(HCV)1b型基因高变区1(HVR1)、非结构5A(NS5A)区准种复杂性及与干扰素(IFN)疗效关系。方法：应用SSCP法检测慢性丙型肝炎患者IFN治疗前后HCV准种的动态变化。结果：治疗前IFN治疗有效组HCV HVR1准种的复杂性(SSCP条带数)明显低于无效组，NS5A区两组无差异。无效组在治疗过程中，大部分患者HVR1 SSCP条带数较治疗前有所减少。结论：HVRl准种的低复杂性预示对IFN治疗产生应答，可以进行疗效预测。NS5A区准种的复杂性与IFN疗效无关，不能用于疗效预测。

Relationship Between Hepatitis C Virus Quasispecies Complexity and Response to Interferon Therapy

Objective: Our purpose was to investigate the relationship between hepatitis C virus (HCV) hypervariable region 1 (HVR1) and nonstructural protein 5A(NS5A) quasispecies complexity and the effectiveness to interferon (IFN) therapy in patients with HCV 1b infection.Methods: Dynamic changes of HCV quasispecies before and after IFN therapy were detected by the method of polymerase chain reaction-mediated single-strand conformation polymorphism (SSCP).Results: The complexity of HVR1 quasispecies in responders was less than that in nonresponders before IFN treatment,whereas the complexity of NS5A quasispecies was not different between the 2 groups. Numbers of SSCP bands of HVR1 were decreased after IFN therapy in most patients.Conclusion: The low complexity of HCV HVR1 quasispecies indicates the effectiveness of IFN therapy in patients with HCV 1b infection. No correlation exists between the complexity of NS5A quasispecies and the effectiveness of IFN therapy.