环氧化酶2抑制剂对大鼠术后腹腔粘连的影响

目的 研究环氧化酶2(COX-E)的表达与术后粘连发生的关系,探讨选择性COX-E抑制剂塞来昔布在预防术后腹腔粘连中的作用.方法 健康SD大鼠50只,随机分为5组(,n=lO),分别为阳性对照组、透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组、阴性对照组.每组于造模术后第8天和第15天随机处死大鼠各5只,肉眼观察评估腹腔粘连程度,并取创伤部位肠管及腹膜Western-blot法检测组织COX-2蛋白含量.结果 透明质酸钠组、塞来昔布小剂量组、塞来昔布大剂量组腹腔粘连程度以及创伤部位组织COX-2表达明显减低,与阳性对照组相比差异有统计学意义(P<0.05).透明质酸钠组与各塞来昔布组相比差异有统计学意义(P<0.05).结论 COX-2选择性抑制剂塞来昔布通过降低创伤部位组织COX-2表达能减轻术后腹腔粘连.

Effects of celecoxib a COX-2 inhibitor on postoperative abdominal adhesion in rats

Objective To study the corelation between the expression of COX-2 and postoperative adhesions and to determine the effect of COX-2 selective inhibitor,Celecoxib,on postoperative adhesion formation. Methods Fifty SD rats were randomly assigned to five groups each consisting of 10 rats,Study groups were as follows:(A)positive control group,(B)sodiumhyahronate group,(C)low dose Celecoxib group,(D)high dose Celecexib group,(E)negative control group,Five rats in each group were treated accordingly for consecutive 8 days and 15 days respectively and sacrificed,After treatment,intra-abdminal adhesions were scored using a standard method.The adhesions tissure and injured peritonaeum were subjected to Westem-blotting to detect the expression of COX-2. Results The level of postoperative adhesions and expression of COX-2 of sodiumhyahronate group、low dose Celecoxib group、high dose Celecoxib group were lower than that of positive control group(P<0.05),Sediumhyahronate treatment group was different formthe two Celecoxib treated groups(P<0.05).Conclusions Selective COX-2COX-2.mechanism,provides durable inhibition of intra-abdominal adhesions through an antiangiongenic COX-2 mechanism.