| 流式细胞术检测药物抗铜绿假单胞菌效应 |
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| 引用本文： | 周蓓,潘伟光,杜冀晖.流式细胞术检测药物抗铜绿假单胞菌效应[J].中国药房,2007,18(13):986-988. |
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| 作者姓名： | 周蓓 潘伟光 杜冀晖 |
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| 作者单位： | 深圳市第六人民医院,深圳市,518052 |
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| 摘 要： | 目的:探索建立流式细胞术(FCM)检测哌拉西林/他唑巴坦抗铜绿假单胞菌效应的方法。方法:以碘化丙啶(PI)为荧光染料,用FCM检测铜绿假单胞菌标准菌株ATCC27853在不同浓度哌拉西林/他唑巴坦(TZPP)作用下各管平均荧光强度(MFI),并寻找其最佳试验条件。结果:TZPP浓度范围为0·125～64μg·mL-1时,细菌MFI值随药物浓度的升高而呈上升趋势。结论:FCM可用于检测药物抗铜绿假单胞菌的效应,并为进一步的药敏试验提供基础。
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| 关 键 词： | 流式细胞术 哌拉西林/他唑巴坦 铜绿假单胞菌 检测 抗菌效应 |
| 文章编号： | 1001-0408(2007)13-0986-03 |
| 修稿时间： | 2006年6月21日 |
Detection of Anti-Pseudomonas Aeruginosa Effect of Drug by Flow Cytometry |
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| ZHOU Bei,PAN Weiguang,DU Jihui.Detection of Anti-Pseudomonas Aeruginosa Effect of Drug by Flow Cytometry[J].China Pharmacy,2007,18(13):986-988. |
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| Authors: | ZHOU Bei PAN Weiguang DU Jihui |
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| Abstract: | OBJECTIVE: To establish a flow cytometry (FCM) method for the detection of anti-Pseudomonas aeruginosa (PA) effect of Piperacilline/Tazobactam. METHODS: With propidium iodide (PI) taken as fluorchrome, the mean fluorescence intensity (MFI) of Pseudomonas aeruginosa standard strain ATCC 27853 in different concentrations of Piperacilline/Tazobactam were detected by FCM, and the experiment condition was optimized. RESULTS: At a concentration range of 0.125～64μg·mL-1 for TZPP, MFI value of bacteria showed an increase tendency with the increase of blood drug concentration. CONCLUSION: FCM can be used to detect the anti-PA effect of drugs and provide the basis for further drug susceptibility test. |
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| Keywords: | Flow cytometry Piperacillin/Tazobactam Pseudomonas aeruginosa Detection Antibacterial effect |
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