神经组织特异表达绿色荧光蛋白转基因小鼠的脑组织形态观察

目的 建立神经组织特异表达绿色荧光蛋白的转基因小鼠,为神经系统的形态学观察提供可以荧光示踪的工具动物.方法 把增强型绿色荧光蛋白(EGFP) 因插入血小板源性生长因(PDGF)B-链启动子下游构建转基因载体,用显微注射的方法建立转基因C57BL/6J小鼠.PCR鉴定转基因小鼠的基因表型,对阳性转基因小鼠的脑组织进行矢状面冷冻切片,分别进行HE染色,显微镜观察组织结构,荧光体视镜及荧光显微镜观察绿色荧光蛋白(GFP)在神经组织的表达.结果 在8个首建品系中筛选出1个神经组织高表达绿色荧光蛋白的转基因小鼠系.观察到绿色荧光蛋白在大脑皮层、海马、丘脑、小脑及脑干等部位表达.结论 建立了稳定遗传的神经组织特异表达绿色荧光蛋白转基因小鼠品系,为神经系统的生理学及病理学研究提供了可以荧光示踪的模型动物.

THE MORPHOLOGICAL ANALYSIS OF BRAIN TISSUE FROM TRANSGENIC MOUSE WITH EXPRESSED GREEN FLUORESCENT PROTEIN IN NERVOUS TISSUES

Objective To establish transgenic mice with nervous tissues specifically expressing green fluorescent protein and to make an animal model for the study of nervous system by fluorescent imaging. Methods The transgenic plasmid was constructed by inserting the EGFP gene under the control of PDGF B-chain promoter and transgenic C57BL/6J mice were established by microinjection method. The transgenic mice were genotyped by PCR. The total of 48 pairs of freezing sections of transgenic mice were prepared in the sagittal plane, and every two adjacent sections were observed under microscope and compared with HE staining and fluorescent imaging. Results One transgenic line with high levels of green fluorescent protein expression was detected from eight transgenic lines. Green fluorescent protein expression was observed in the tissues of cerebrum, hippocampus, thalamencephalon, cerebellum and brain stem. Conclusion A transgenic mouse line was established with nervous tissues specifically expressing green fhcore sunt protein. The results suggested that it will be an available animal model for the study of nervous tissues by fluorescent imaging.