多病毒感染时T细胞亚群、NK与LAK细胞活性的变化及其意义

目的：探讨多病毒感染时细胞免疫和ＮＫ，ＬＡＫ细胞活性的变化及其意义。方法：以１种或多种病毒抗原，或ＩｇＭ抗体阳性的住院患儿６１例为观察对象，其中单病毒组３８例，多病毒组２３例，另设对照组３０例，采用ＥＬＩＳＡ法测定柯萨奇病毒（ＣＶＢ）抗原和ＩｇＭ抗体、ＥＢ病毒（ＥＢＶ）ＩｇＭ，巨细胞病毒（ＣＶＭ）ＩｇＭ、乙肝病毒（ＨＢＶ）血清标志物５项，单克隆抗体的间接ＡＢＣ免疫组化法测定Ｔ细胞亚群；＾３Ｈ－Ｔｄ

Changes of T lymphocyte subpopulation,NK and LAK cell activities in multiviral infection

Objective:To investigate the changes of T cell immunity,NK and LAK cell activities and their significance in multiviral infection. Methods:Sixty-one cases with positive virus antigen or IgM antibody of single or several virus (38 single virus, 23 several virus ) and 30 controls were enrolled in the study. We detected CVB-Ag, CVB-IgM, EBV-IgM, CMV-IgM and HBV serumal markers using ELISA method, T lymphocyte subpopulation using OKT method, NK and LAK cell activities using H-TdR method. Results: (1) CD4/CD8 ratio, NK cell activity and LAK cell activity were obviously decreased in early viral infection stage and the level of CD8 obviously increased (P<0. 01 ); (2) Both LAK cell activity and the CD4/CD8 ratio improved in convalesence of single viral infection (P<0. 01,P<0. 05 ); (3) In convalescence of multiviral infection, NK and LAK cell activities decreased more markedly than the activities in early viral infection stage (P<0. 05, P