重铬酸钾比色法测定盐酸小檗碱片的含量

 目的研究盐酸小檗碱片中盐酸小檗碱的含量测定方法，并与中国药典方法的测定结果比较，评价两种方法的相关性。方法用重铬酸钾比色法测定含量，以421 nm为测定波长，0.2 mg·mL^-1的基准重铬酸钾溶液作对照液。结果盐酸小檗碱在4.0～28.0μg·mL^-1之间呈良好的线性关系，相关系数r=0.9999，平均回收率为100.05%(n=5),RSD为0.43%。比较本法和中国药典方法的测定结果，相关性显著。结论本法简便、准确、重现性好，可用于盐酸小檗碱的含量测定。尤适于生产过程中的快速分析。

Determination of the content of berberine hydrochloride tablets by potassium dichromate colormetric method

OBJECTIVE　To develop an assay for the determination of berberine hydrochloride in berberine hydrochloride tablets,and to compare the results measured by colormetric method with the assay described in Chinese Pharmacopoeia 1995 for assessing the correlation of the two methods.METHODS　Potassium dichromate colormetric method was selected to determine the content, and the 0.2 mg*mL-1Potassium dichromate was used as the reference solution. The absorbency was measured at 421 nm.RESULTS　The calibration curve for berberine hydrochloride was linear within the concentration range of 4.0～28.0 μg*mL-1(r=0.9999).The average recovery was 100.05%(n=5),and the RSD was 0.43%.The analytical results for berberine hydrochloride between the two methods was found to be correlated.CONCLUSION　The method was proved to be simple, precise and reproduciable. It can be used for the quantitative determination of berberine hydrochloride. It is especially practicable for rapid analysis in production.