石头鱼毒素胶体金检测试纸条的研制 |
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引用本文: | 周志杰,刘珠果,戴秋云. 石头鱼毒素胶体金检测试纸条的研制[J]. 军事医学, 2016, 0(11). DOI: 10.7644/j.issn.1674-9960.2016.11.013 |
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作者姓名: | 周志杰 刘珠果 戴秋云 |
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作者单位: | 军事医学科学院生物工程研究所,北京,100071 |
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基金项目: | 国家863计划资助项目(SS2013AA090204) |
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摘 要: | 目的:研制石头鱼毒素(neoverrucotoxin,stonefish toxin,neoVTX)的胶体金检测试纸条,建立一种该毒素的快速检测方法。方法采用柠檬酸三钠还原法制备胶体金溶液,以neoVTX马血清抗体用于胶体金标记,并将该抗体固定于硝酸纤维素膜作为捕获抗体,利用双抗体夹心法原理制备胶体金检测试纸条。结果制备的检测试纸条对该毒素检测灵敏度为50 ng/ml,该试纸与牛血清白蛋白、卵清蛋白及水母毒素等蛋白无反应,具有较好的特异性。结论成功研制了石头鱼毒素胶体金检测试纸,该试纸条灵敏度高,特异性好,可用于该毒素的快速检测。
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关 键 词: | 石头鱼毒素 neoVTX 胶体金试纸 检测 |
Preparation of colloidal gold test strips for neoVTX |
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Abstract: | Objective To develop a colloidal gold test strip for neoVTX , and establish a quick detection method of this toxin.Methods Colloidal gold was prepared by trisodium citrate reduction method and labeled with neoVTX horse antibody.The antibody was also immobilized on cellulose nitrate membrane as a capture antibody.Colloidal gold test strips were prepared using double antibody sandwich method .Results The detection sensitivity of the test strip was 50 ng/ml of neoVTX toxin with high specificity and repeatability .Bovine serum albumin , ovalbumin and jellyfish toxin did not interfere with the detection .Conclusion A colloidal gold test strip for the detection of neoVTX was successfully developed .Due to its high sensitivity and good specificity , this method can be used for its rapid detection. |
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Keywords: | stonefish toxin neoVTX colloidal gold test strip detection |
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