四逆汤配方颗粒煎煮前后6种成分的含量变化研究

目的：探讨四逆汤配方颗粒在煎煮过程中6种成分的动态变化状况。方法：采用高效液相色谱法，以Agilent ZORBAX SB-C18（4.6 mm×250 mm，5μm）为色谱柱，柱温：25℃，检测波长：235 nm，流动相：乙腈-0.1%甲酸水（5 mmol·L-1乙酸铵），梯度洗脱，流速：1.0 mL·min-1，进样量：15μL。结果：随着煎煮时间延长，甘草苷、甘草酸铵的含量增加，新乌头碱、6-姜烯酚的含量先增加后降低，苯甲酰次乌头碱、6-姜酚的含量无明显变化。结论：初步揭示四逆汤中三味中药配方颗粒经合并煎煮，6种化学成分含量有了变化，为中药配方颗粒的临床使用提供了参考。

Content comparison of six constituents in Sini decoction formula granules before and after decocting

Ob jective: To probe the decoctional changes of six constituents in Sini decoction formula granules. Methods: An HPLC method was used for the determination with the following conditions, column:Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5μm); temperature of column: 25℃; detective wavelength: 235 nm; mobile phase: acetonitrile-0.1% formic acid aqueous solution (5 mmol·L-1 ammonium acetate), with gra-dient elution; flow speed: 1.0 mL·min-1; injection volume: 15μL. Results: With the decoction time pro-longed, the contents of liquiritin and glycyrrhizic acid increased; the contents of mesaconitine and 6-shogaol increased at first and then decreased; but the contents of benzoylhypaconitine and 6-gingerol had no obvious changes. Conclusion: From the perspective of chemical composition, it has been preliminary revealed the dynamic changes of six constituents in Sini decoction formula granules in the decoction pro-cess. And it provides scientific basis for clinical application of herbal formula granules.