复方熊去氧胆酸口服液对大鼠肝纤维化的防治作用

目的：观察复方熊去氧胆酸口服液（C-UDCA）对四氯化碳（CCl4）致大鼠肝纤维化的防治作用。方法：采用皮下注射40%CCl4-花生油溶液制备肝纤维化模型，检测经口给予C-UDCA后对大鼠肝功能指标、肝纤维化各项生化指标及肝组织病理形态学的影响。结果：C-UDCA各剂量组能显著减轻CCl4导致的肝脂肪变性、肝细胞损伤和纤维组织增生。与UDCA相比，C-UDCA-H能显著降低血清ALT、AST、HA、PCⅢ、CⅣ、LN及肝组织Hyp含量，显著升高血清Alb、A/G、肝组织GSH含量；C-UDCA-M亦能显著降低血清HA、LN 含量。结论：C-UDCA对CCl4致大鼠肝纤维化具有一定的防治作用，且C-UDCA-H、C-UDCA-M的作用优于UDCA。

Effects of Compound Ursodeoxycholic Acid oral liquid on Hepatic Fibrosis in Rats

Objective: The study was designed to observe the effects of C-UDCA on hepatic fibrosis in rats. Methods: The effects of C-UDCA on experimental hepatic fibrosis were observed in model rats in-duced by carbon tetrachloride. Intragastric administration of different doses of C-UDCA was followed by determination of serum ALT, AST, HA, LN, PCⅢ and CⅣ and live GSH and Hyp. Histopathological changes in liver tissues were also examined by HE staining. Results: In comparison with the model group, all doses of C-UDCA significantly reduced the levels of serum ALT, AST, HA, PCⅢ, CⅣand LN, and the content of Hyp in liver tissues. Moreover, the contents of GSH in liver tissues were elevated. Compared with UDCA, C-UDCA-H remarkably decreased the levels of serum ALT, AST, HA, PCⅢ, CⅣ and LN and the contents of Hyp in liver tissues, while the contents of GSH in liver tissues were significantly ele-vated. Meanwhile, C-UDCA-M also obviously lowered the levels of serum HA and LN. Conclusion: Our data suggested that C-UDCA could significantly improve liver function from fibrogenesis and deday the liv-er pathological changes. Furthermore, C-UDCA-H and C-UDCA-M were more effective than UDCA.