亚洲副溶血弧菌临床菌株的基于碱基序列和肽链序列的多位点序列分型研究

目的：了解来自亚洲的副溶血弧菌临床菌株的群组结构和克隆复合体构成。方法在 pubMLST 公共数据中筛选具有完整 ST 型及 pST 型的来自亚洲的副溶血弧菌临床菌株数据，进行亚群分析和克隆复合体分析，并分别构建基于 ST 型和 pST 型的最小生成树。结果从数据库中共筛选到具有 ST 型及 pST 型的来自亚洲的副溶血弧菌临床菌株数据341条，共含有157个 ST 型，以 ST3型为最多。其中有214条菌株数据来自中国（包括港台地区），覆盖了133个 ST 型，其中仍以 ST3型为最多。利用 eBURST 软件对数据进行分析，共发现了17个组，94个单体。STRUCTURE 软件分析显示，来自亚洲的副溶血弧菌临床菌株的适宜亚群数为7，各亚群内的样品平均距离为0．9113。结论来自亚洲的副溶血弧菌临床菌株具有高度多态性，可细分为7个亚群，MLST 分型时以 ST3型为多，AA-MLST 分型时以 pST2型为主。

Population analysis of clinical Vibrio parahaemolyticus strains from Asia based on multilocus sequence typing and AA-multilocus sequence typing

Objective To find out more about the population structure and clonal complex of clinical Vibrio parahaemolyticus strains in Asia.Methods Clinical Vibrio parahaemolyticus strains data were screened in Asia with complete ST and pST types from PubMLST public database,their subgroup and complex were analyzed,and the minimum spanning tree based on ST and pST types respectively was completed.Results From the database,341 items of ST and pST types of Asian clinical Vibrio parahaemolyticus strains were screened,including 157 ST,most of which were of ST3 type.Totally 214 items of data came from China (including Hong Kong and Taiwan),and covered 133 ST,most of which were of ST3 type.eBURST software was used and 17 groups and 94 singletons were found.Software STRUCTURE analysis showed that the appropriate subset number of clinical V.parahaemolyticus strains in Asia was 7,and that the average distance between samples in each subgroup was 0.9113.Conclusion Clinical V.parahaemolyticus strains in Asia show high diversity and can be subdivided into seven subgroups.ST3 type is dominating when multilocus sequence typing(MLST)is used and pST2 type is the majority by AA-MLST typing.