氮卓斯汀对豚鼠哮喘的拮抗作用及相关机制

目的:研究组胺H1受体拮抗剂——氮卓斯汀(azelastine,AZT)对豚鼠哮喘的拮抗作用,探讨氮卓斯汀拮抗哮喘的可能机制。方法:以卵白蛋白致敏豚鼠,制备哮喘模型,通过豚鼠引喘潜伏期和跌倒率的变化评价氮卓斯汀对哮喘的拮抗作用;分别计数外周血和支气管肺泡灌洗液(BALF)中嗜酸性粒细胞的数量;采用ELISA法检测血清和BALF中IL-4和IL-5的质量浓度。结果:与模型组相比,氮卓斯汀能显著延长致敏豚鼠的引喘潜伏期,降低跌倒率;氮卓斯汀显著减少哮喘豚鼠血清和BALF中嗜酸性粒细胞的数量,降低血清和BALF中IL-4和IL-5的水平。结论:降低IL-4和IL-5的水平,减少血中嗜酸性粒细胞的数量及其在气道中的浸润,可能是组胺H1受体拮抗剂——氮卓斯汀拮抗哮喘的机制之一。

Studies on Antagonistic Effect and Relevant Mechanisms of Azelastine in Asthma of Guinea Pigs

Objective：To study the antagonistic effect and the relevant mechanisms of azelastine on asthma of guinea pigs.Methods：Experimental asthma model of guinea pigs was induced by ovalbumin in vivo.The antagonistic effect of azelastine on asthma of guinea pigs was evaluated by the changes of asthmatic latency and tumble ratio.The number of eosinophils in blood and bronchoalveolar lavage fluid（BALF）was counted.The content of IL-4 and IL-5 in serum and BALF was detected by enzyme-linked immunosorbent assay（ELISA）.Results：Azelastine prolonged the asthmatic latency and reduced tumble ratio in asthmatic guinea pigs.The number of eosinophils and the levels of IL-4 and IL-5 in serum and BALF of asthmatic guinea pigs treated with azelastine decreased significantly.Conclusion：Lowering the levels of IL-4 and IL-5,and reducing the number of eosinophils in blood and BALF may be one of the mechanisms for azelastine to antagonize asthma.