单纯性肥胖者血清胆汁酸谱研究

目的探讨单纯性肥胖者血清胆汁酸谱的特征,为阐明胆汁酸引发的能量代谢相关机制和探寻新的肥胖生物治疗靶点提供研究依据。方法利用超高效液相色谱-串联质谱(UPLC-MS/MS)联用方法检测体检人群中10名表观正常对照者、10例超重者和10例单纯性肥胖者血清胆汁酸谱的特征。结果表观正常对照组、超重组和单纯性肥胖组血清中14种胆汁酸亚组分均有表达,其中的石胆酸(LCA)、牛磺胆酸(TCA)、牛磺石胆酸(TLCA)和牛磺熊脱氧胆酸(TUDCA)的表达水平最低;14种胆汁酸亚组分在各组间的差异均无统计学意义(P>0.05);单纯性肥胖组的胆汁酸谱中以甘氨鹅脱氧胆酸(GCDCA)和牛磺鹅脱氧胆酸(TCDCA)增高为主;超重组及单纯性肥胖组血清游离型胆汁酸(FBA)和结合胆汁酸(CBA)水平稍低于表观正常对照组,但差异无统计学意义(P>0.05)。结论单纯性肥胖者血清胆汁酸谱中以GCDCA和TCDCA增高为主,但各种胆汁酸亚组分的表达水平与表观正常者相同。

Research of serum bile acid profiles in patients with simple obesity

Objective To investigate the expression characteristics of serum bile acid profiles in patients with simple obesity, further to understand the effect of bile acids inducing signaling on human body energy metabolism, and to provide experimental evidence and clues on searching potential biotherapy target. Methods Ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） assay was used to test serum bile acid profiles of 10 healthy subjects, 10 subjects with overweight and 10 patients with simple obesity. Results A total of 14 bile acid subgroups were detected in all sera from healthy, overweight and simple obesity groups, and lithoeholic acid （ LCA）, taurocholie acid （TCA）, taurolithocholie acid （TLCA） and tauroursodeoxycholic acid （TUDCA） had lowest expression in all groups. The expressions of all different 14 bile acid subgroups had no statistical significance（P 〉0.05 ）. The levels of glycochenodeoxycholic acid （GCDCA） and tauroehenodeoxycholic acid （TCDCA） increased markedly in serum bile acid （FBA） profiles of simple obesity group. Both free bile acids （CBA） and conjugated bile acids from overweight and simple obesity groups were lower than those from healthy group （ P 〉 0.05 ）. Conclusions The levels of GCDCA and TCDCA increase markedly in serum bile acid profiles of patients with simple obesity, but the expressions of all different 14 bile acid subgroups have no significant difference with those of healthy subjects.