Human papillomavirus genotypes distribution in cervical samples from Uruguayan women |
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| Authors: | Viviana Ramas Santiago Mirazo Sylvia Bonilla Laura Mendoza Olga Lago Jorge Basiletti Joaquin González Maria Alejandra Picconi Juan Arbiza |
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| Institution: | 1. Virology Department, Faculty of Science. University of the Republic. Montevideo, Uruguay;2. Cytology Laboratory, Health Care Service, Municipality of Montevideo, Uruguay;3. Department of Public Health and Epidemiology, Research Institute of Health Science, University of Asunción, Asunción, Paraguay;4. Institute for Clinical Effectiveness and Health Policy, Buenos Aires, Argentina |
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| Abstract: | Persistent infection with high‐risk human papillomavirus (HPV) causes cervical preneoplasic lesions and invasive cervical cancer. This study evaluated the prevalence and distribution of HPV genotypes in cervical exfoliated cells from Uruguayan women. Five hundred sixty‐eight cervical specimens were examined by PCR using MY09/11 primer set, and were genotyped by restriction enzyme digestion (RFLP). Some of the samples which remained undetermined were reanalyzed by PGMY PCR combined with reverse line blot hybridization. Overall, about 42% of samples were positive for HPV; 96% in high‐grade squamous intraepithelial lesion, 66% in low‐grade squamous intraepithelial lesion, 15% in atypical squamous cells of undetermined significance, and 19% in samples negative for intraepithelial lesion or malignancy. HPV 16 was the most commonly found genotype, followed by HPV 68 and 58. Within low risk—HPV genotypes 6, 61, and 11 were the most frequent. This is the first cross‐sectional study, accounting for prevalence and genotype distribution of HPV in Uruguayan women. J. Med. Virol. 85:845–851, 2013. © 2013 Wiley Periodicals, Inc. |
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| Keywords: | HPV prevalence squamous intraepithelial lesion cross‐sectional study |
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