Exogenous phytoestrogenic molecule icaritin incorporated into a porous scaffold for enhancing bone defect repair |
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| Authors: | Xin‐Luan Wang Xin‐Hui Xie Ge Zhang Shi‐Hui Chen Dong Yao Kai He Xiao‐Hong Wang Xin‐Sheng Yao Yang Leng Kwok‐Pui Fung Kwok‐Sui Leung Ling Qin |
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| Institution: | 1. Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong, China;2. Translational Medicine Research and Development Center, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China;3. The Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, China;4. Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing, China;5. Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou, China;6. Department of Physics and the Institute of Nano Science and Technology, The Hong Kong University of Science and Technology, Hong Kong, China;7. School of Basic Medical Sciences, The Chinese University of Hong Kong, Hong Kong, China |
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| Abstract: | This study was designed to develop a bioactive scaffold to enhance bone defect repair in steroid‐associated osteonecrosis (SAON). Icaritin, a metabolite of the herb Epimedium, has been identified as an angiogenic and osteogenic phytomolecule. Icaritin was homogenized into poly lactic‐co‐glycolic acid/tricalcium phosphate (PLGA/TCP) to form an icaritin‐releasing porous composite scaffold (PLGA/TCP/icaritin) by fine‐spinning technology. In vitro, high performance liquid chromatography was used to determine the release of icaritin during degradation of PLGA/TCP/icaritin. The osteogenic effects of PLGA/TCP/icaritin were evaluated using rat bone marrow mesenchymal stem cells (BMSCs). In vivo, the osteogenic effect of PLGA/TCP/icaritin was determined within a bone tunnel after core decompression in SAON rabbits and angiography within scaffolds was examined in rabbit muscle pouch model. In vitro study confirmed the sustainable release of icaritin from PLGA/TCP/icaritin with the bioactive scaffold promoting the proliferation and osteoblastic differentiation of rat BMSCs. In vivo study showed that PLGA/TCP/icaritin significantly promoted new bone formation within the bone defect after core decompression in SAON rabbits and enhanced neovascularization in the rabbit muscle pouch experiment. In conclusion, PLGA/TCP/icaritin is an innovative local delivery system that demonstrates sustainable release of osteogenic phytomolecule icaritin enhancing bone repair in an SAON rabbit model. The supplement of scaffold materials with bioactive phytomolecule(s) might improve treatment efficiency in challenging orthopedic conditions. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31:164–172, 2012 |
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| Keywords: | PLGA/TCP/icaritin porous scaffold osteogenesis angiogenesis SAON |
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