Reproducibility study for free‐breathing measurements of pyruvate metabolism using hyperpolarized 13C in the heart |
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Authors: | Angus Z Lau Albert P Chen Jennifer Barry John J Graham William Dominguez‐Viqueira Nilesh R Ghugre Graham A Wright Charles H Cunningham |
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Institution: | 1. Department of Medical Biophysics, Sunnybrook Health Sciences Centre, University of Toronto, , Toronto, Ontario, Canada;2. Imaging Research, Sunnybrook Health Sciences Centre, , Toronto, Ontario, Canada;3. GE Healthcare, , Toronto, Ontario, Canada;4. Division of Cardiology, St. Michael's Hospital, University of Toronto, , Toronto, Ontario, Canada |
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Abstract: | Spatially resolved images of hyperpolarized 13C substrates and their downstream products provide insight into real‐time metabolic processes occurring in vivo. Recently, hyperpolarized 13C pyruvate has been used to characterize in vivo cardiac metabolism in the rat and pig, but accurate and reproducible measurements remain challenging due to the limited period available for imaging as well as physiological motion. In this article, time‐resolved cardiac‐ and respiratory‐gated images of 1‐13C] pyruvate, 1‐13C] lactate, and 13C bicarbonate in the heart are acquired without the need for a breathhold. The robustness of these free‐breathing measurements is demonstrated using the time‐resolved data to produce a normalized metric of pyruvate dehydrogenase and lactate dehydrogenase activity in the heart. The values obtained are reproducible in a controlled metabolic state. In a 60‐min ischemia/reperfusion model, significant differences in hyperpolarized bicarbonate and lactate, normalized using the left ventricular pyruvate signal, were detected between scans performed at baseline and 45 min after reperfusion. The sequence is anticipated to improve quantitative measurements of cardiac metabolism, leading to feasible validation studies using fewer subjects, and potentially improved diagnosis, serial monitoring, and treatment of cardiac disease in patients. Magn Reson Med 69:1063–1071, 2013. © 2012 Wiley Periodicals, Inc. |
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Keywords: | cardiac metabolism hyperpolarized 13C pyruvate |
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