肾移植群体反应性抗体检测641例分析

目的探讨群体反应性抗体检测(PRA)对于肾移植的意义。方法采用ELISA方法对641例肾移植患者进行PRA检测:术前570例、术后71例。结果570例术前检测PRA,阴性490例,术后发生急性排斥反应35例;弱阳性68例,术后发生急性排斥反应36例;12例阳性术后检测仍为阳性,发生急性排斥反应10例。71例术后检测PRA,阴性59例,发生急性排斥反应3例;阳性12例,发生急性排斥反应7例。结论肾移植患者术前检测PRA有助于减少排斥反应的发生。

Detection of panel-reactive antibody before and after renal transplantation: analysis of 641 cases

Objective To explore the significance of the panel-reactive antibody (PRA) detection in renal transplantation. Methods Enzyme-linked immunosorbent assay (ELISA) was used to measure the PRA levels in 641 patients with renal trans-plantation, of whom 570 were examined preoperatively while 71 postoperatively. Results Of the 570 cases examined preop-eratively for PRA levels, 490 cases were negative of PRA in which acute rejection occurred in 35 cases (7.14%) after the transplantation, while in the 68 cases with slightly positive PRA as detected preoperatively, acute rejection occurred in 36 cas-es (52.9%). Among the 12 cases with positive PRA assay both before and after the operation, acute rejection took place in 10 cases (83.3%). As for the 71 patients receiving postoperative PRA detection, 59 were PRA-negative, among whom acute re-jection occurred in only 3 cases (5.08%), while 7 (58.3%) out of the 12 PRA-positive experienced episodes of acute rejection. Conclusion PRA-positive recipients of the renal transplantation have higher risks of acute rejection of the graft, and PRA de-tection both before and after the operation may be of great importance.